Welcome, here's your workspace. Feel free to edit this title to serve your research.

From 2012.igem.org

(Difference between revisions)
(Created page with "http://www.blackwellpublishing.com/genecloning/pdfs/chapter7.pdf")
(Prokaryotic System, Protein Characterization, and Yeast Genome Integration)
Line 1: Line 1:
-
http://www.blackwellpublishing.com/genecloning/pdfs/chapter7.pdf
+
'''Yeast System''' <br/>
 +
<li>[http://www.google.com/url?q=http%3A%2F%2Fbiochemie.web.med.uni-muenchen.de%2FYeast_Biol%2F04%2520Yeast%2520Molecular%2520Techniques.pdf&sa=D&sntz=1&usg=AFQjCNEnj2JbQh6nxUnja5PpZ3Vch5Jy6g Yeast Vectors 1]
 +
<li>[http://www.google.com/url?q=http%3A%2F%2Fwww.blackwellpublishing.com%2Fgenecloning%2Fpdfs%2Fchapter7.pdf&sa=D&sntz=1&usg=AFQjCNEl8DNQGCHr4QH-Rb7zmn6oSAZm2A Yeast Vectors 2]
 +
<li>[http://www.google.com/url?q=http%3A%2F%2Fwww.genetics.org%2Fcontent%2F122%2F1%2F19.full.pdf&sa=D&sntz=1&usg=AFQjCNHIPhHrNJnRxDusytVTLpu59shf0Q A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces ceratisiae]<br/><br/>
 +
 
 +
'''Yeast Integrative Plasmid Vectors (YIp)'''<br/>
 +
<li>[http://partsregistry.org/Part:BBa_K300001 AL1 and GAL10 (galactose catabolism) selection]
 +
<li>[http://partsregistry.org/Part:BBa_K165061 TRP1 selection]<br/><br/>
 +
 
 +
'''Prokaryotic System'''<br/><br/>
 +
 
 +
''Promoters''
 +
<li>[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 BBa-J23100 (strong)]
 +
<li>[http://partsregistry.org/Part:BBa_J23108 BBa-J23108 (medium)]
 +
<li>[http://partsregistry.org/Part:BBa_J23109 BBa-J23109 (weak)]<br/>
 +
''Terminators''
 +
<li>[http://partsregistry.org/Part:BBa_B0015 BBa-B0015]<br/>
 +
''RBS''
 +
<li>[http://partsregistry.org/Part:BBa_B0034 Constitutive]<br/><br/>
 +
 
 +
'''Secretion'''
 +
<li>[http://www.google.com/url?q=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fgene%2F3990775&sa=D&sntz=1&usg=AFQjCNFRRVh0vsU3zS9AzvSGko6WIQe0Rg HlyA Gene Sequence]
 +
<li>[http://www.google.com/url?q=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F2693460&sa=D&sntz=1&usg=AFQjCNGNhjPJSpqeHsdjbo29PuRXFmWmFA A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium]
 +
<li>[http://www.google.com/url?q=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F1791766&sa=D&sntz=1&usg=AFQjCNFNdr3RpmJXoTQ69ehSmrLMYMRp8Q Analysis of the haemolysin transport process through the secretion from Escherichia coli of PCM, CAT or beta-galactosidase fused to the Hly C-terminal signal domain]
 +
<li>[http://www.google.com/url?q=http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F2277639&sa=D&sntz=1&usg=AFQjCNHlr8EyE98_b0wRCRqamhBzJpAw8g Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins]<br/><br/>
 +
 
 +
'''Kumamolisin Info'''
 +
 
 +
<li>57 kDa (beta-galactosidase: 540 kDa; [http://www.mpbio.com/detailed_info.php?family_key=02104939] source)
 +
<li>1701 bp
 +
<li>567 aa
 +
<li>MSDMEKPWKEGEEARAVLQGHARAQAPQAVDKGPVAGDERMAVTVVLRRQRAGELAAHVERQAAIAPHAREHLKREAFAASHGASLDDFAELRRFADAHGLALDRANVAAGTAVLSGPVDAINRAFGVELRHFDHPDGSYRSYLGEVTVPASIAPMIEAVLGLDTRPVARPHFRMQRRAEGGFEARSQAAAPTAYTPLDVAQAYQFPEGLDGQGQCIAIIELGGGYDEASLAQYFASLGVPAPQVVSVSVDGASNQPTGDPSGPDGEVELDIEVAGALAPGAKFAVYFAPNTDAGFLDAITTAIHDPTLKPSVVSISWGGPEDSWTSAAIAAMNRAFLDAAALGVTVLAAAGDSGSTDGEQDGLYHVDFPAASPYVLACGGTRLVASGGRIAQETVWNDGPDGGATGGGVSRIFPLPAWQEHANVPPSANPGASSGRGVPDLAGNADPATGYEVVIDGEATVIGGTSAVAPLFAALVARINQKLGKAVGYLNPTLYQLPADVFHDITEGNNDIANRAQIYQAGPGWDPCTGLGSPIGVRLLQALLPSASQPQPGSTENLYFQSGALE

Revision as of 17:35, 18 June 2012

Yeast System

  • Yeast Vectors 1
  • Yeast Vectors 2
  • A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces ceratisiae

    Yeast Integrative Plasmid Vectors (YIp)
  • AL1 and GAL10 (galactose catabolism) selection
  • TRP1 selection

    Prokaryotic System

    Promoters
  • BBa-J23100 (strong)
  • BBa-J23108 (medium)
  • BBa-J23109 (weak)
    Terminators
  • BBa-B0015
    RBS
  • Constitutive

    Secretion
  • HlyA Gene Sequence
  • A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium
  • Analysis of the haemolysin transport process through the secretion from Escherichia coli of PCM, CAT or beta-galactosidase fused to the Hly C-terminal signal domain
  • Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins

    Kumamolisin Info
  • 57 kDa (beta-galactosidase: 540 kDa; [1] source)
  • 1701 bp
  • 567 aa
  • MSDMEKPWKEGEEARAVLQGHARAQAPQAVDKGPVAGDERMAVTVVLRRQRAGELAAHVERQAAIAPHAREHLKREAFAASHGASLDDFAELRRFADAHGLALDRANVAAGTAVLSGPVDAINRAFGVELRHFDHPDGSYRSYLGEVTVPASIAPMIEAVLGLDTRPVARPHFRMQRRAEGGFEARSQAAAPTAYTPLDVAQAYQFPEGLDGQGQCIAIIELGGGYDEASLAQYFASLGVPAPQVVSVSVDGASNQPTGDPSGPDGEVELDIEVAGALAPGAKFAVYFAPNTDAGFLDAITTAIHDPTLKPSVVSISWGGPEDSWTSAAIAAMNRAFLDAAALGVTVLAAAGDSGSTDGEQDGLYHVDFPAASPYVLACGGTRLVASGGRIAQETVWNDGPDGGATGGGVSRIFPLPAWQEHANVPPSANPGASSGRGVPDLAGNADPATGYEVVIDGEATVIGGTSAVAPLFAALVARINQKLGKAVGYLNPTLYQLPADVFHDITEGNNDIANRAQIYQAGPGWDPCTGLGSPIGVRLLQALLPSASQPQPGSTENLYFQSGALE
    Retrieved from "http://2012.igem.org/Welcome,_here%27s_your_workspace._Feel_free_to_edit_this_title_to_serve_your_research."