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From 2012.igem.org

I. Order Sequencing Primers from Keck

• Primers were ordered with the name/sequence in the following table:

Name	Tm	Length (bp)	%GC	Sequence
p2f_seq	61.86	27	40%	5'-GCGGATAACAATTACTAGAGAAAGAGG-3'
p2r_seq	59.45	21	52%	5'-CTCCATACCTCATCACTCCTC-3'
p3f_seq	56.14	22	41%	5'-GTAATATTGAGGAGTGATGAGG-3'
p3r_seq	61.76	22	50%	5'-CCTCTTTCTTAATTACGCCCCG-3'
p4f_seq	60.35	25	40%	5'-GGCGTAATTAAGAAAGAGGAGAAAG-3'
p4r_seq	64.01	27	40%	5'-GACTGAGCCTTTCGTTTTATTTGATGC-3'

• See Project Page for more info