Test if gluten has been metabolized.

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This page is for Steven's research.

http://eprints.uniss.it/5145/1/Forteschi_M_Study_of_peptidases_involved.pdf 33-mer peptide: LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF

http://www.sciencedirect.com/science/article/pii/0009898190900824 Detection and estimation of the barley prolamin content of beer and malt to assess their suitability for patients with coeliac disease

http://www.pnas.org/content/99/10/6603.full A peptide-based fluorescence resonance energy transfer assay for Bacillus anthracis lethal factor protease

1)substrate (peptide) synthesis using ABI Model 433A peptide synthesizer 2)labeling with N-hydroxysuccinimidyl esters. Reactions were carried out on a 20–100-μmol resin scale with a 1–10-fold excess of the label in a minimal volume (1–3 ml) of N-methylpyrrolidinone overnight. 3)Incorporation of the two reporters was accomplished by first labeling the N terminus. The second reporter was then introduced by selectively removing the Lys(Mtt)-protecting group and reaction with the label as described above.