http://2012.igem.org/wiki/index.php?title=Test_if_gluten_has_been_metabolized.&feed=atom&action=historyTest if gluten has been metabolized. - Revision history2024-03-29T01:41:16ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Test_if_gluten_has_been_metabolized.&diff=32102&oldid=prevSdenham at 16:08, 12 July 20122012-07-12T16:08:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://mcl1.ncifcrf.gov/wlo_pubs/261.pdf enzyme structure and kinetics for Kumamolisin</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://mcl1.ncifcrf.gov/wlo_pubs/261.pdf enzyme structure and kinetics for Kumamolisin</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">http://www.jbc.org/content/282/36/26401.full.pdf+html Structure and function of EnvZ, specifically looking at the histidine kinase domain and the HAMP domain.</ins></div></td></tr>
</table>Sdenhamhttp://2012.igem.org/wiki/index.php?title=Test_if_gluten_has_been_metabolized.&diff=20220&oldid=prevTrips145 at 22:56, 27 June 20122012-06-27T22:56:34Z<p></p>
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</table>Trips145http://2012.igem.org/wiki/index.php?title=Test_if_gluten_has_been_metabolized.&diff=15516&oldid=prevSdenham at 17:33, 20 June 20122012-06-20T17:33:33Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.sciencedirect.com/science/article/pii/0009898190900824</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.sciencedirect.com/science/article/pii/0009898190900824</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Detection and estimation of the barley prolamin content of beer and malt to assess their suitability for patients with coeliac disease</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Detection and estimation of the barley prolamin content of beer and malt to assess their suitability for patients with coeliac disease</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-a study confirming that beer contains levels of gluten antigens harmful to Celiacs. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-a study confirming that beer contains levels of gluten antigens harmful to Celiacs. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.sciencemag.org/content/297/5590/2275.full</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.sciencemag.org/content/297/5590/2275.full</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Structural Basis for Gluten Intolerance in Celiac Sprue</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Structural Basis for Gluten Intolerance in Celiac Sprue</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-The antigenicity of gluten seems to be due to proline and glutamine rich peptides, the most prevalent one being the 33-mer listed above. The sequence seems to be fairly conserved across barley and wheat.Three distinct patient-specific T cell epitopes identified previously in T cell proliferation assays are present in this peptide, namely, PFPQPQLPY, PQPQLPYPQ (three copies), and PYPQPQLPY (two copies).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-The antigenicity of gluten seems to be due to proline and glutamine rich peptides, the most prevalent one being the 33-mer listed above. The sequence seems to be fairly conserved across barley and wheat.Three distinct patient-specific T cell epitopes identified previously in T cell proliferation assays are present in this peptide, namely, PFPQPQLPY, PQPQLPYPQ (three copies), and PYPQPQLPY (two copies).</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.sunrisescience.com/Files/pTEF-MF.pdf</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.sunrisescience.com/Files/pTEF-MF.pdf</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pTEF-MF constitutive yeast vector marked for secretion</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pTEF-MF constitutive yeast vector marked for secretion</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-MF-alpha secretion tag for yeast is used in this plasmid. I used the plasmid map to get the tag's sequence and BLASTed it. A 100% match confirmed that the sequence is present in yeast. We will attach this to the N-terminus of Kumamolisin, hopefully enabling the protein to be secreted in yeast.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-MF-alpha secretion tag for yeast is used in this plasmid. I used the plasmid map to get the tag's sequence and BLASTed it. A 100% match confirmed that the sequence is present in yeast. We will attach this to the N-terminus of Kumamolisin, hopefully enabling the protein to be secreted in yeast.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.jbc.org/content/263/13/6209.full.pdf</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.jbc.org/content/263/13/6209.full.pdf</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Prepro-a-factor Has a Cleavable Signal Sequence</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Prepro-a-factor Has a Cleavable Signal Sequence</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-MF-alpha signal sequence is attached to the animo-terminus of MF-alpha and cleaved in the golgi. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-MF-alpha signal sequence is attached to the animo-terminus of MF-alpha and cleaved in the golgi. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC391546/pdf/pnas00616-0033.pdf Alphafactor-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC391546/pdf/pnas00616-0033.pdf Alphafactor-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>directed synthesis and secretion of mature foreign proteins</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>in Saccharomyces cerevisiae</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">-The peptide mating pheromone alpha-factor is efficiently secreted into the medium. Plasmids with the secretion factor and foreign proteins were transformed into yeast. Yeast cells transformed with some of these plasmids efficiently synthesize, process, and secrete into the medium the mature biologically active foreign proteins.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.springerlink.com/content/l2115622t21t728w/fulltext.pdf Engineering of protein secretion in yeast:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.springerlink.com/content/l2115622t21t728w/fulltext.pdf Engineering of protein secretion in yeast:</div></td></tr>
</table>Sdenhamhttp://2012.igem.org/wiki/index.php?title=Test_if_gluten_has_been_metabolized.&diff=15496&oldid=prevSdenham at 16:55, 20 June 20122012-06-20T16:55:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://eprints.uniss.it/5145/1/Forteschi_M_Study_of_peptidases_involved.pdf</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://eprints.uniss.it/5145/1/Forteschi_M_Study_of_peptidases_involved.pdf</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>33-mer peptide: LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>33-mer peptide: LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.sciencedirect.com/science/article/pii/0009898190900824</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.sciencedirect.com/science/article/pii/0009898190900824</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Detection and estimation of the barley prolamin content of beer and malt to assess their suitability for patients with coeliac disease</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Detection and estimation of the barley prolamin content of beer and malt to assess their suitability for patients with coeliac disease</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">-a study confirming that beer contains levels of gluten antigens harmful to Celiacs. </ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.sciencemag.org/content/297/5590/2275.full</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.sciencemag.org/content/297/5590/2275.full</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Structural Basis for Gluten Intolerance in Celiac Sprue</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Structural Basis for Gluten Intolerance in Celiac Sprue</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">-The antigenicity of gluten seems to be due to proline and glutamine rich peptides, the most prevalent one being the 33-mer listed above. The sequence seems to be fairly conserved across barley and wheat.Three distinct patient-specific T cell epitopes identified previously in T cell proliferation assays are present in this peptide, namely, PFPQPQLPY, PQPQLPYPQ (three copies), and PYPQPQLPY (two copies).</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.sunrisescience.com/Files/pTEF-MF.pdf</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.sunrisescience.com/Files/pTEF-MF.pdf</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pTEF-MF constitutive yeast vector marked for secretion</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pTEF-MF constitutive yeast vector marked for secretion</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-MF<ins class="diffchange diffchange-inline">-alpha secretion tag for yeast is used in this plasmid</ins>. <ins class="diffchange diffchange-inline">I used the plasmid map to get the tag's sequence and BLASTed it. A 100% match confirmed that the sequence is present in yeast. We will attach this to the N</ins>-<ins class="diffchange diffchange-inline">terminus of Kumamolisin, hopefully enabling the protein to be secreted in </ins>yeast<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">http://www.sunrisescience.com/Files/pGAL</del>-MF.<del class="diffchange diffchange-inline">pdf</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">pGAL</del>-<del class="diffchange diffchange-inline">MF inducible </del>yeast <del class="diffchange diffchange-inline">vector marked for secretion</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.jbc.org/content/263/13/6209.full.pdf</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.jbc.org/content/263/13/6209.full.pdf</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Prepro-a-factor Has a Cleavable Signal Sequence</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Prepro-a-factor Has a Cleavable Signal Sequence</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">-MF-alpha signal sequence is attached to the animo-terminus of MF-alpha and cleaved in the golgi. </ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://synbio.org.uk/dna-assembly/guidetogibsonassembly.html Gibson assembly</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://synbio.org.uk/dna-assembly/guidetogibsonassembly.html Gibson assembly</div></td></tr>
</table>Sdenhamhttp://2012.igem.org/wiki/index.php?title=Test_if_gluten_has_been_metabolized.&diff=15024&oldid=prevSdenham at 22:23, 19 June 20122012-06-19T22:23:59Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://onlinelibrary.wiley.com/doi/10.1002/jms.1667/full Identification of N-glycosylation in prolyl endoprotease from Aspergillus niger and evaluation of the enzyme for its possible application in proteomics</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://onlinelibrary.wiley.com/doi/10.1002/jms.1667/full Identification of N-glycosylation in prolyl endoprotease from Aspergillus niger and evaluation of the enzyme for its possible application in proteomics</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">http://mcl1.ncifcrf.gov/wlo_pubs/261.pdf enzyme structure and kinetics for Kumamolisin</ins></div></td></tr>
</table>Sdenhamhttp://2012.igem.org/wiki/index.php?title=Test_if_gluten_has_been_metabolized.&diff=13370&oldid=prevSdenham at 21:32, 15 June 20122012-06-15T21:32:12Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.springerlink.com/content/l2115622t21t728w/fulltext.pdf Engineering of protein secretion in yeast:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.springerlink.com/content/l2115622t21t728w/fulltext.pdf Engineering of protein secretion in yeast:</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">http://ajpgi.physiology.org/content/291/4/G621.long Highly efficient gluten degradation with a newly identified prolyl endoprotease: implications for celiac disease</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">http://onlinelibrary.wiley.com/doi/10.1002/jms.1667/full Identification of N-glycosylation in prolyl endoprotease from Aspergillus niger and evaluation of the enzyme for its possible application in proteomics</ins></div></td></tr>
</table>Sdenhamhttp://2012.igem.org/wiki/index.php?title=Test_if_gluten_has_been_metabolized.&diff=13000&oldid=prevSdenham at 22:50, 14 June 20122012-06-14T22:50:44Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>in Saccharomyces cerevisiae</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>in Saccharomyces cerevisiae</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">http://www.springerlink.com/content/l2115622t21t728w/fulltext.pdf Engineering of protein secretion in yeast:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">strategies and impact on protein production</ins></div></td></tr>
</table>Sdenhamhttp://2012.igem.org/wiki/index.php?title=Test_if_gluten_has_been_metabolized.&diff=12960&oldid=prevSdenham at 22:10, 14 June 20122012-06-14T22:10:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://synbio.org.uk/dna-assembly/guidetogibsonassembly.html Gibson assembly</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://synbio.org.uk/dna-assembly/guidetogibsonassembly.html Gibson assembly</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC391546/pdf/pnas00616-0033.pdf Alphafactor-</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">directed synthesis and secretion of mature foreign proteins</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">in Saccharomyces cerevisiae</ins></div></td></tr>
</table>Sdenhamhttp://2012.igem.org/wiki/index.php?title=Test_if_gluten_has_been_metabolized.&diff=11855&oldid=prevSdenham at 16:09, 12 June 20122012-06-12T16:09:25Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Prepro-a-factor Has a Cleavable Signal Sequence</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Prepro-a-factor Has a Cleavable Signal Sequence</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">http://synbio.org.uk/dna-assembly/guidetogibsonassembly.html Gibson assembly</ins></div></td></tr>
</table>Sdenhamhttp://2012.igem.org/wiki/index.php?title=Test_if_gluten_has_been_metabolized.&diff=10886&oldid=prevSdenham at 19:48, 8 June 20122012-06-08T19:48:52Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pTEF-MF constitutive yeast vector marked for secretion</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pTEF-MF constitutive yeast vector marked for secretion</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pGAL-MF inducible yeast vector marked for secretion</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pGAL-MF inducible yeast vector marked for secretion</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Prepro-a-factor Has a Cleavable Signal Sequence</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Prepro-a-factor Has a Cleavable Signal Sequence</div></td></tr>
</table>Sdenham