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Test if gluten has been metabolized.
From 2012.igem.org
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2)labeling with N-hydroxysuccinimidyl esters. Reactions were carried out on a 20–100-μmol resin scale with a 1–10-fold excess of the label in a minimal volume (1–3 ml) of N-methylpyrrolidinone overnight. | 2)labeling with N-hydroxysuccinimidyl esters. Reactions were carried out on a 20–100-μmol resin scale with a 1–10-fold excess of the label in a minimal volume (1–3 ml) of N-methylpyrrolidinone overnight. | ||
3)Incorporation of the two reporters was accomplished by first labeling the N terminus. The second reporter was then introduced by selectively removing the Lys(Mtt)-protecting group and reaction with the label as described above. | 3)Incorporation of the two reporters was accomplished by first labeling the N terminus. The second reporter was then introduced by selectively removing the Lys(Mtt)-protecting group and reaction with the label as described above. | ||
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| + | http://www.sciencemag.org/content/297/5590/2275.full | ||
| + | Structural Basis for Gluten Intolerance in Celiac Sprue | ||
Revision as of 21:36, 7 June 2012
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This page is for Steven's research.
http://eprints.uniss.it/5145/1/Forteschi_M_Study_of_peptidases_involved.pdf 33-mer peptide: LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF
http://www.sciencedirect.com/science/article/pii/0009898190900824 Detection and estimation of the barley prolamin content of beer and malt to assess their suitability for patients with coeliac disease
http://www.pnas.org/content/99/10/6603.full A peptide-based fluorescence resonance energy transfer assay for Bacillus anthracis lethal factor protease
1)substrate (peptide) synthesis using ABI Model 433A peptide synthesizer 2)labeling with N-hydroxysuccinimidyl esters. Reactions were carried out on a 20–100-μmol resin scale with a 1–10-fold excess of the label in a minimal volume (1–3 ml) of N-methylpyrrolidinone overnight. 3)Incorporation of the two reporters was accomplished by first labeling the N terminus. The second reporter was then introduced by selectively removing the Lys(Mtt)-protecting group and reaction with the label as described above.
http://www.sciencemag.org/content/297/5590/2275.full Structural Basis for Gluten Intolerance in Celiac Sprue
