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2024-03-29T09:03:53Z
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<h2>3 Cell transformation</h2><br />
<p>&nbsp;</p><br />
<p>3.1 Remove competent cells on ice to thaw.</p><br />
<p>&nbsp;</p><br />
<p>3.2 Add 1-10 μl of DNA to the cells. </p><br />
<p>&nbsp;</p><br />
<p>3.3 Incubate on ice for 20 min.</p><br />
<p>&nbsp;</p><br />
<p>3.4 Spread onto LB + antibiotic agar plate. Incubate overnight at 37℃. </p><br />
<p>&nbsp;</p><br />
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Lvjiajun