http://2012.igem.org/wiki/index.php?title=Team:ZJU-China/brainstorm2.htm&feed=atom&action=historyTeam:ZJU-China/brainstorm2.htm - Revision history2024-03-29T07:03:34ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:ZJU-China/brainstorm2.htm&diff=292079&oldid=prevAireenMei at 23:34, 26 October 20122012-10-26T23:34:36Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="justify">Reference:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="justify">Reference:</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align="justify">1. <del class="diffchange diffchange-inline">"Synthetic biology: synchronized bacterial clock"</del><<del class="diffchange diffchange-inline">/p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align="justify">1. <<ins class="diffchange diffchange-inline">a href</ins>="http://www.nature.com/nature/journal/v463/n7279/full/463301a.html<ins class="diffchange diffchange-inline">" target</ins>="<ins class="diffchange diffchange-inline">_blank</ins>">"<ins class="diffchange diffchange-inline">Synthetic biology: </ins>synchronized <ins class="diffchange diffchange-inline">bacterial </ins>clock"<ins class="diffchange diffchange-inline"></a></ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><p align</del>="<del class="diffchange diffchange-inline">justify">(</del>http://www.nature.com/nature/journal/v463/n7279/full/463301a.html<del class="diffchange diffchange-inline">)</p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align="justify"><ins class="diffchange diffchange-inline">2. <a href="</ins>http://www.nature.com/nature/journal/v463/n7279/abs/nature08753.html<ins class="diffchange diffchange-inline">" target</ins>="<ins class="diffchange diffchange-inline">_blank</ins>">"<ins class="diffchange diffchange-inline">A synchronized quorum of genetic clock</ins>"<ins class="diffchange diffchange-inline"></a></ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><p align</del>="<del class="diffchange diffchange-inline">justify</del>"><del class="diffchange diffchange-inline">2. </del>"<del class="diffchange diffchange-inline">A </del>synchronized <del class="diffchange diffchange-inline">quorum of genetic </del>clock"</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align="justify"><ins class="diffchange diffchange-inline">3. <a href="</ins>http://www.nature.com/nature/journal/v481/n7379/full/481033a.html<ins class="diffchange diffchange-inline">.html" target="_blank">"bacteria collaborate to sense arsenic"</a></ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align="justify"><del class="diffchange diffchange-inline">(</del>http://www.nature.com/nature/journal/v463/n7279/abs/nature08753.html<del class="diffchange diffchange-inline">)</p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br/></ins></div></td></tr>
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</table>AireenMeihttp://2012.igem.org/wiki/index.php?title=Team:ZJU-China/brainstorm2.htm&diff=286760&oldid=prevMingchen at 17:07, 26 October 20122012-10-26T17:07:31Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="justify">We found that Hasty and colleagues have constructed a network of genes and proteins in E.coli that acts as a molecular clock and can be synchronized across a population of the bacteria. The mechanism can be described briefly as follows.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="justify">We found that Hasty and colleagues have constructed a network of genes and proteins in E.coli that acts as a molecular clock and can be synchronized across a population of the bacteria. The mechanism can be described briefly as follows.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="justify">A promoter(PluxI)drives the production of LuxI, an enzyme that synthesizes the quorumsensing signal acyl-homoserine lactone (AHL). Another PluxI control ths the production of AiiA, a protein that catalyses the degradation of AHL, and a third PluxI triggers the synthesis of a variant of green fluorescent protein called yemGFP. An AHL receptor, LuxR, is constitutively expressed. The authors combined these components to form an autoinducng circuit (AHL activates LuxR, and the AHL-LuxR complex induces PluxI-driven luxI transcription and yemGFP production) with a time-delayed negative feedback loop (the AHL-LuxR complex induces PluxI-driven production of AiiA, which degrades AHL). The result was a population of bacteria that produce synchronized pulses of fluorescence, coordinated by quorum sensing. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="justify">A promoter(PluxI)drives the production of LuxI, an enzyme that synthesizes the quorumsensing signal acyl-homoserine lactone (AHL). Another PluxI control ths the production of AiiA, a protein that catalyses the degradation of AHL, and a third PluxI triggers the synthesis of a variant of green fluorescent protein called yemGFP. An AHL receptor, LuxR, is constitutively expressed. The authors combined these components to form an autoinducng circuit (AHL activates LuxR, and the AHL-LuxR complex induces PluxI-driven luxI transcription and yemGFP production) with a time-delayed negative feedback loop (the AHL-LuxR complex induces PluxI-driven production of AiiA, which degrades AHL). The result was a population of bacteria that produce synchronized pulses of fluorescence, coordinated by quorum sensing. </p></div></td></tr>
</table>Mingchenhttp://2012.igem.org/wiki/index.php?title=Team:ZJU-China/brainstorm2.htm&diff=187188&oldid=prevLvjiajun at 05:54, 26 September 20122012-09-26T05:54:13Z<p></p>
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</table>Lvjiajunhttp://2012.igem.org/wiki/index.php?title=Team:ZJU-China/brainstorm2.htm&diff=187145&oldid=prevLvjiajun at 05:49, 26 September 20122012-09-26T05:49:48Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Synchronized bacterial clock</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Synchronized bacterial clock</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Human orchestrate their activities in different places because of their synchronizing clocks. Isn't it cool if bacteria can coordinate their molecular timepieces in the same way as we human do?</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>>Human orchestrate their activities in different places because of their synchronizing clocks. Isn't it cool if bacteria can coordinate their molecular timepieces in the same way as we human do?</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>We found that Hasty and colleagues have constructed a network of genes and proteins in E.coli that acts as a molecular clock and can be synchronized across a population of the bacteria. The mechanism can be described briefly as follows.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>>We found that Hasty and colleagues have constructed a network of genes and proteins in E.coli that acts as a molecular clock and can be synchronized across a population of the bacteria. The mechanism can be described briefly as follows.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><img src="https://static.igem.org/mediawiki/2012/2/23/Zju_brainstorm3.jpg" width="500px"><p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>><img src="https://static.igem.org/mediawiki/2012/2/23/Zju_brainstorm3.jpg" width="500px"><p <ins class="diffchange diffchange-inline">align="justify"</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>A promoter(PluxI)drives the production of LuxI, an enzyme that synthesizes the quorumsensing signal acyl-homoserine lactone (AHL). Another PluxI control ths the production of AiiA, a protein that catalyses the degradation of AHL, and a third PluxI triggers the synthesis of a variant of green fluorescent protein called yemGFP. An AHL receptor, LuxR, is constitutively expressed. The authors combined these components to form an autoinducng circuit (AHL activates LuxR, and the AHL-LuxR complex induces PluxI-driven luxI transcription and yemGFP production) with a time-delayed negative feedback loop (the AHL-LuxR complex induces PluxI-driven production of AiiA, which degrades AHL). The result was a population of bacteria that produce synchronized pulses of fluorescence, coordinated by quorum sensing. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>>A promoter(PluxI)drives the production of LuxI, an enzyme that synthesizes the quorumsensing signal acyl-homoserine lactone (AHL). Another PluxI control ths the production of AiiA, a protein that catalyses the degradation of AHL, and a third PluxI triggers the synthesis of a variant of green fluorescent protein called yemGFP. An AHL receptor, LuxR, is constitutively expressed. The authors combined these components to form an autoinducng circuit (AHL activates LuxR, and the AHL-LuxR complex induces PluxI-driven luxI transcription and yemGFP production) with a time-delayed negative feedback loop (the AHL-LuxR complex induces PluxI-driven production of AiiA, which degrades AHL). The result was a population of bacteria that produce synchronized pulses of fluorescence, coordinated by quorum sensing. </p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>(From "Synthetic biology: synchronized bacterial clock")</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>>(From "Synthetic biology: synchronized bacterial clock")</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>We expect to apply the network to medical field. Since the network can make an oscillation, it can be used to release medicine periodically. What is more, we wish to add another sensor in the network. The sensor can sense a signal which is released because of a certain disease, and thus the cells can sense the signal and start the oscillation which releases the medicine periodically. For instance, when a man hasdiabetes, the high density blood sugar will start an insulin-releasing oscillation.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>>We expect to apply the network to medical field. Since the network can make an oscillation, it can be used to release medicine periodically. What is more, we wish to add another sensor in the network. The sensor can sense a signal which is released because of a certain disease, and thus the cells can sense the signal and start the oscillation which releases the medicine periodically. For instance, when a man hasdiabetes, the high density blood sugar will start an insulin-releasing oscillation.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Reference:</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>>Reference:</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>1. "Synthetic biology: synchronized bacterial clock"</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>>1. "Synthetic biology: synchronized bacterial clock"</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>(http://www.nature.com/nature/journal/v463/n7279/full/463301a.html)</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>>(http://www.nature.com/nature/journal/v463/n7279/full/463301a.html)</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>2. "A synchronized quorum of genetic clock"</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>>2. "A synchronized quorum of genetic clock"</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>(http://www.nature.com/nature/journal/v463/n7279/abs/nature08753.html)</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>>(http://www.nature.com/nature/journal/v463/n7279/abs/nature08753.html)</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>3. "bacteria collaborate to sense arsenic"</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>>3. "bacteria collaborate to sense arsenic"</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>(http://www.nature.com/nature/journal/v481/n7379/full/481033a.html)</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">align="justify"</ins>>(http://www.nature.com/nature/journal/v481/n7379/full/481033a.html)</p></div></td></tr>
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</table>Lvjiajunhttp://2012.igem.org/wiki/index.php?title=Team:ZJU-China/brainstorm2.htm&diff=171261&oldid=prevLvjiajun at 13:23, 25 September 20122012-09-25T13:23:11Z<p></p>
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</table>Lvjiajunhttp://2012.igem.org/wiki/index.php?title=Team:ZJU-China/brainstorm2.htm&diff=170829&oldid=prevLvjiajun at 13:05, 25 September 20122012-09-25T13:05:43Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 13:05, 25 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We found that Hasty and colleagues have constructed a network of genes and proteins in E.coli that acts as a molecular clock and can be synchronized across a population of the bacteria. The mechanism can be described briefly as follows.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We found that Hasty and colleagues have constructed a network of genes and proteins in E.coli that acts as a molecular clock and can be synchronized across a population of the bacteria. The mechanism can be described briefly as follows.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>A promoter(PluxI)drives the production of LuxI, an enzyme that synthesizes the quorumsensing signal acyl-homoserine lactone (AHL). Another PluxI control ths the production of AiiA, a protein that catalyses the degradation of AHL, and a third PluxI triggers the synthesis of a variant of green fluorescent protein called yemGFP. An AHL receptor, LuxR, is constitutively expressed. The authors combined these components to form an autoinducng circuit (AHL activates LuxR, and the AHL-LuxR complex induces PluxI-driven luxI transcription and yemGFP production) with a time-delayed negative feedback loop (the AHL-LuxR complex induces PluxI-driven production of AiiA, which degrades AHL). The result was a population of bacteria that produce synchronized pulses of fluorescence, coordinated by quorum sensing. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>A promoter(PluxI)drives the production of LuxI, an enzyme that synthesizes the quorumsensing signal acyl-homoserine lactone (AHL). Another PluxI control ths the production of AiiA, a protein that catalyses the degradation of AHL, and a third PluxI triggers the synthesis of a variant of green fluorescent protein called yemGFP. An AHL receptor, LuxR, is constitutively expressed. The authors combined these components to form an autoinducng circuit (AHL activates LuxR, and the AHL-LuxR complex induces PluxI-driven luxI transcription and yemGFP production) with a time-delayed negative feedback loop (the AHL-LuxR complex induces PluxI-driven production of AiiA, which degrades AHL). The result was a population of bacteria that produce synchronized pulses of fluorescence, coordinated by quorum sensing. </p></div></td></tr>
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<h3>Synchronized bacterial clock</h3><br />
<p>Human orchestrate their activities in different places because of their synchronizing clocks. Isn't it cool if bacteria can coordinate their molecular timepieces in the same way as we human do?</p><br />
<p>We found that Hasty and colleagues have constructed a network of genes and proteins in E.coli that acts as a molecular clock and can be synchronized across a population of the bacteria. The mechanism can be described briefly as follows.</p><br />
<br />
<p><img src="https://static.igem.org/mediawiki/2012/2/23/Zju_brainstorm3.jpg" width="80%"><p><br />
<br />
<p>A promoter(PluxI)drives the production of LuxI, an enzyme that synthesizes the quorumsensing signal acyl-homoserine lactone (AHL). Another PluxI control ths the production of AiiA, a protein that catalyses the degradation of AHL, and a third PluxI triggers the synthesis of a variant of green fluorescent protein called yemGFP. An AHL receptor, LuxR, is constitutively expressed. The authors combined these components to form an autoinducng circuit (AHL activates LuxR, and the AHL-LuxR complex induces PluxI-driven luxI transcription and yemGFP production) with a time-delayed negative feedback loop (the AHL-LuxR complex induces PluxI-driven production of AiiA, which degrades AHL). The result was a population of bacteria that produce synchronized pulses of fluorescence, coordinated by quorum sensing. </p><br />
<p>(From "Synthetic biology: synchronized bacterial clock")</p><br />
<br />
<p>We expect to apply the network to medical field. Since the network can make an oscillation, it can be used to release medicine periodically. What is more, we wish to add another sensor in the network. The sensor can sense a signal which is released because of a certain disease, and thus the cells can sense the signal and start the oscillation which releases the medicine periodically. For instance, when a man hasdiabetes, the high density blood sugar will start an insulin-releasing oscillation.</p><br />
<br />
<br />
<p>Reference:</p><br />
<p>1. "Synthetic biology: synchronized bacterial clock"</p><br />
<p>(http://www.nature.com/nature/journal/v463/n7279/full/463301a.html)</p><br />
<p>2. "A synchronized quorum of genetic clock"</p><br />
<p>(http://www.nature.com/nature/journal/v463/n7279/abs/nature08753.html)</p><br />
<p>3. "bacteria collaborate to sense arsenic"</p><br />
<p>(http://www.nature.com/nature/journal/v481/n7379/full/481033a.html)</p><br />
<br />
</body></html></div>Lvjiajun