Team:Yale

From 2012.igem.org

(Difference between revisions)
(Projects)
Line 20: Line 20:
==Projects==
==Projects==
 +
 +
[REVIEW BEFORE FREEZE]
The Yale 2012 iGEM Team is currently pursuing:
The Yale 2012 iGEM Team is currently pursuing:

Revision as of 21:06, 3 October 2012


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions
You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Yale logo.png

Projects

[REVIEW BEFORE FREEZE]

The Yale 2012 iGEM Team is currently pursuing:

MAGE in a naturally competent organism: Multiplex Automated Genome Engineering (MAGE), a technique for rapidly generating genomic diversity using the recombination ability of the λ-phage ssDNA-binding protein β, has to date been introduced only in E. coli. These cells must be transformed via electroporation for each MAGE cycle to facilitate efficient uptake of mutagenic oligonucleotides, but this process kills a significant portion of otherwise viable cells. We aim to introduce MAGE in naturally competent bacteria such as B. subtilis and A. baylyi by testing homologs of β and uniquely optimizing the technique within each species to avoid the death/recovery time inherent with electroporation. In addition, we are developing CAD-MAGE to predict the diversity produced by MAGE.


Your team picture
Team Yale