Team:XMU-China/protocolsindex

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       <th>Contents<span id="hide"><a href="#" onClick="MM_changeProp('indextd4','','display','none','TD');MM_changeProp('indextd1','','display','none','TD');MM_changeProp('indextd2','','display','none','TD');MM_changeProp('indextd3','','display','none','TD');MM_changeProp('hide','','display','none','SPAN');MM_changeProp('indextd5','','display','none','TD');MM_changeProp('indextd6','','display','none','TD');MM_changeProp('indextd7','','display','none','TD');MM_changeProp('indextd8','','display','none','TD');MM_changeProp('indextd9','','display','none','TD');MM_changeProp('indextd10','','display','none','TD');MM_changeProp('indextd11','','display','none','TD');MM_changeProp('indextd12','','display','none','TD');MM_changeProp('indextd13','','display','none','TD');MM_changeProp('indextd14','','display','none','TD');MM_changeProp('indextd15','','display','none','TD');MM_changeProp('show','','display','block','SPAN');MM_changeProp('indextd16','','display','none','TD');MM_changeProp('indextd17','','display','none','TD');MM_changeProp('indextd18','','display','none','TD');MM_changeProp('indextd19','','display','none','TD');MM_changeProp('indextd20','','display','none','TD');MM_changeProp('indextd21','','display','none','TD');MM_changeProp('indextd22','','display','none','TD');MM_changeProp('indextd23','','display','none','TD');MM_changeProp('indextd24','','display','none','TD');MM_changeProp('indextd25','','display','none','TD')">[hide]</a></span>
       <th>Contents<span id="hide"><a href="#" onClick="MM_changeProp('indextd4','','display','none','TD');MM_changeProp('indextd1','','display','none','TD');MM_changeProp('indextd2','','display','none','TD');MM_changeProp('indextd3','','display','none','TD');MM_changeProp('hide','','display','none','SPAN');MM_changeProp('indextd5','','display','none','TD');MM_changeProp('indextd6','','display','none','TD');MM_changeProp('indextd7','','display','none','TD');MM_changeProp('indextd8','','display','none','TD');MM_changeProp('indextd9','','display','none','TD');MM_changeProp('indextd10','','display','none','TD');MM_changeProp('indextd11','','display','none','TD');MM_changeProp('indextd12','','display','none','TD');MM_changeProp('indextd13','','display','none','TD');MM_changeProp('indextd14','','display','none','TD');MM_changeProp('indextd15','','display','none','TD');MM_changeProp('show','','display','block','SPAN');MM_changeProp('indextd16','','display','none','TD');MM_changeProp('indextd17','','display','none','TD');MM_changeProp('indextd18','','display','none','TD');MM_changeProp('indextd19','','display','none','TD');MM_changeProp('indextd20','','display','none','TD');MM_changeProp('indextd21','','display','none','TD');MM_changeProp('indextd22','','display','none','TD');MM_changeProp('indextd23','','display','none','TD');MM_changeProp('indextd24','','display','none','TD');MM_changeProp('indextd25','','display','none','TD')">[hide]</a></span>
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       <td id="indextd1"><li id="indexli"><a id="indexa" href="#_Toc01">1.&nbsp; <strong> General Protocols</strong></a></li> </td>
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       <td id="indextd1"><li id="indexli"><a id="indexa" href="#_Toc01"><strong>1 General protocols</strong></a></li> </td>
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       <td id="indextd12"><li id="indexli"><a id="indexa" href="#_Toc12"><strong>2.&nbsp;&nbsp;Characterization</strong></a></li> </td>
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       <td id="indextd2"><li id="indexli"><a id="indexa" href="#_Toc02"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.1&nbsp;Stock solution</strong></a></li></td>
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       <td id="indextd20"><li id="indexli"><a id="indexa" href="#_Toc20"><strong>3.&nbsp;&nbsp; Immobilization</strong></a></li> </td>
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      <td id="indextd3"><li id="indexli"><a id="indexa" href="#_Toc03"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.2&nbsp;Preparation of Competent BL21</strong></a><a href="#_Toc03"> </a></li></td>
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      <td id="indextd4"><li id="indexli"><a id="indexa" href="#_Toc04"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.3&nbsp;Transformation</strong></a></li> </td>
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      <td id="indextd5"><li id="indexli"><a id="indexa" href="#_Toc05"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.4&nbsp;Plasmid Purification</strong></a></li> </td>
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      <td id="indextd6"><li id="indexli"><a id="indexa" href="#_Toc06"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.5&nbsp;Reaction system of restriction endonuclease</strong></a></li> </td>
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      <td id="indextd7"><li id="indexli"><a id="indexa" href="#_Toc07"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.6&nbsp;Standard  BioBrick Assembly</strong></a></li> </td>
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      <td id="indextd8"><li id="indexli"><a id="indexa" href="#_Toc08"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.7&nbsp;Suffix  Insertion</strong></a></li> </td>
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      <td id="indextd9"><li id="indexli"><a id="indexa" href="#_Toc09"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.8&nbsp;Ligation</strong></a></li> </td>
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      <td id="indextd10"><li id="indexli"><a id="indexa" href="#_Toc10"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.9&nbsp;Restriction  analysis</strong></a></li> </td>
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      <td id="indextd11"><li id="indexli"><a id="indexa" href="#_Toc11"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.10&nbsp;Gel  Extraction</strong></a></li> </td>
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      <td id="indextd12"><li id="indexli"><a id="indexa" href="#_Toc12"><strong>2 Characterization</strong></a></li> </td>
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      <td id="indextd13"><li id="indexli"><a id="indexa" href="#_Toc13"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.1&nbsp;Fluorescence Measurements </strong></a></li> </td>
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      <td id="indextd14"><li id="indexli"><a id="indexa" href="#_Toc14"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2&nbsp;Protein electrophoresis</strong></a></li> </td>
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      <td id="indextd15"><li id="indexli"><a id="indexa" href="#_Toc15"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.1&nbsp;Preparation of Samples</strong></a></li> </td>
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      <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc16"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.2&nbsp;Manufacture Albumen Gel</strong></a></li> </td>
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      <td id="indextd17"><li id="indexli"><a id="indexa" href="#_Toc17"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.3&nbsp;Electrophoresis</strong></a></li> </td>
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      <td id="indextd18"><li id="indexli"><a id="indexa" href="#_Toc18"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.4&nbsp;Dyeing (Colloidal Coomassie Brilliant Blue)</strong></a></li> </td>
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      <td id="indextd19"><li id="indexli"><a id="indexa" href="#_Toc19"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.5&nbsp;Scanning</strong></a></li> </td>
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       <td id="indextd20"><li id="indexli"><a id="indexa" href="#_Toc20"><strong>3 Immobilization</strong></a></li> </td>
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      <td id="indextd21"><li id="indexli"><a id="indexa" href="#_Toc21"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.1&nbsp;Prepare Sodiumcellu-losesulfate (NaCS)</strong></a></li> </td>
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      <td id="indextd22"><li id="indexli"><a id="indexa" href="#_Toc22"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.2&nbsp;Prepare microcapsules</strong></a></li> </td>
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      <td id="indextd23"><li id="indexli"><a id="indexa" href="#_Toc23"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.3&nbsp;Immobilize cells into calcium alginate beads</strong></a></li> </td>
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      <td id="indextd24"><li id="indexli"><a id="indexa" href="#_Toc24"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.4&nbsp;Immobilize cells into intra-hollow Ca-alginate capsules</strong></a></li> </td>
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      <td id="indextd25"><li id="indexli"><a id="indexa" href="#_Toc25"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.5&nbsp;Immobilize cells into NaCS-PDMDAAC microcapsules</strong></a></li> </td>
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Revision as of 15:46, 25 September 2012

safetyindex

Contents[hide] [show]
  • 1 General protocols
  •        1.1 Stock solution
  •        1.2 Preparation of Competent BL21
  •        1.3 Transformation
  •        1.4 Plasmid Purification
  •        1.5 Reaction system of restriction endonuclease
  •        1.6 Standard BioBrick Assembly
  •        1.7 Suffix Insertion
  •        1.8 Ligation
  •        1.9 Restriction analysis
  •        1.10 Gel Extraction
  • 2 Characterization
  •        2.1 Fluorescence Measurements
  •        2.2 Protein electrophoresis
  •               2.2.1 Preparation of Samples
  •               2.2.2 Manufacture Albumen Gel
  •               2.2.3 Electrophoresis
  •               2.2.4 Dyeing (Colloidal Coomassie Brilliant Blue)
  •               2.2.5 Scanning
  • 3 Immobilization
  •        3.1 Prepare Sodiumcellu-losesulfate (NaCS)
  •        3.2 Prepare microcapsules
  •        3.3 Immobilize cells into calcium alginate beads
  •        3.4 Immobilize cells into intra-hollow Ca-alginate capsules
  •        3.5 Immobilize cells into NaCS-PDMDAAC microcapsules