Team:XMU-China/protocolsindex

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Revision as of 09:48, 25 September 2012

protocolsindex

Contents[hide] [show]
  • 1 General protocols
    		•
  •        1.1 Stock solution
    		•
  •        1.2 Preparation of Competent BL21
  •        1.3 Transformation
    		•
  •        1.4 Plasmid Purification
    		•
  •        1.5 Reaction system of restriction endonuclease
    		•
  •        1.6 Standard BioBrick Assembly
    		•
  •        1.7 Suffix Insertion
    		•
  •        1.8 Ligation
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  •        1.9 Restriction analysis
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  •        1.10 Gel Extraction
    		•
  • 2 Characterization
    		•
  •        2.1 Fluorescence Measurements
  •        2.2 Protein electrophoresis
    		•
  •               2.2.1 Preparation of Samples
    		•
  •               2.2.2 Manufacture Albumen Gel
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  •               2.2.3 Electrophoresis
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  •               2.2.4 Dyeing (Colloidal Coomassie Brilliant Blue)
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  •               2.2.5 Scanning
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  • 3 Immobilization
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  •        3.1 Prepare Sodiumcellu-losesulfate (NaCS)
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  •        3.2 Prepare microcapsules
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  •        3.3 Immobilize cells into calcium alginate beads
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  •        3.4 Immobilize cells into intra-hollow Ca-alginate capsules
    		•
  •        3.5 Immobilize cells into NaCS-PDMDAAC microcapsules
    		•

    Retrieved from "http://2012.igem.org/Team:XMU-China/protocolsindex"