Team:XMU-China/protocolsindex

From 2012.igem.org

(Difference between revisions)
Line 125: Line 125:
     <tr>
     <tr>
-
       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc25"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.4&nbsp;Immobilize cells into intra-hollow Ca-alginate capsules</strong></a></li> </td>
+
       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc24"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.4&nbsp;Immobilize cells into intra-hollow Ca-alginate capsules</strong></a></li> </td>
     </tr>
     </tr>
       <tr>
       <tr>
-
       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc26"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.5&nbsp;Immobilize cells into NaCS-PDMDAAC microcapsules</strong></a></li> </td>
+
       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc25"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.5&nbsp;Immobilize cells into NaCS-PDMDAAC microcapsules</strong></a></li> </td>
     </tr>
     </tr>

Revision as of 14:21, 23 September 2012

safetyindex

Contents[hide] [show]
  • 1 General protocols
    		•
  •        1.1 Stock solution
    		•
  •        1.2 Preparation of Competent BL21
  •        1.3 Transformation
    		•
  •        1.4 Plasmid Purification
    		•
  •        1.5 Reaction system of restriction endonuclease
    		•
  •        1.6 Standard BioBrick Assembly
    		•
  •        1.7 Suffix Insertion
    		•
  •        1.8 Ligation
    		•
  •        1.9 Restriction analysis
    		•
  •        1.10 Gel Extraction
    		•
  • 2 Characterization
    		•
  •        2.1 Fluorescence Measurements
  •        2.2 Protein electrophoresis
    		•
  •               2.2.1 Preparation of Samples
    		•
  •               2.2.2 Manufacture Albumen Gel
    		•
  •               2.2.3 Electrophoresis
    		•
  •               2.2.4 Dyeing (Colloidal Coomassie Brilliant Blue)
    		•
  •               2.2.5 Scanning
    		•
  • 3 Immobilization
    		•
  •        3.1 Prepare Sodiumcellu-losesulfate (NaCS)
    		•
  •        3.2 Prepare microcapsules
    		•
  •        3.3 Immobilize cells into calcium alginate beads
    		•
  •        3.4 Immobilize cells into intra-hollow Ca-alginate capsules
    		•
  •        3.5 Immobilize cells into NaCS-PDMDAAC microcapsules
    		•

    Retrieved from "http://2012.igem.org/Team:XMU-China/protocolsindex"