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Team:XMU-China/cellimmobilization - Revision history
2024-03-28T10:54:23Z
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FBS at 04:04, 27 October 2012
2012-10-27T04:04:23Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 1:</b> The simple device that we made for cell immobilization culture.</td></tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 1:</b> The simple device that we made for cell immobilization culture.</td></tr></div></td></tr>
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FBS
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FBS at 04:01, 27 October 2012
2012-10-27T04:01:50Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 1:</b> The simple device that we made for cell immobilization culture.</td></tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 1:</b> The simple device that we made for cell immobilization culture.</td></tr></div></td></tr>
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FBS
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FBS at 03:56, 27 October 2012
2012-10-27T03:56:44Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 1:</b> The simple device that we made for cell immobilization culture.</td></tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 1:</b> The simple device that we made for cell immobilization culture.</td></tr></div></td></tr>
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FBS
http://2012.igem.org/wiki/index.php?title=Team:XMU-China/cellimmobilization&diff=287595&oldid=prev
Youbin at 18:22, 26 October 2012
2012-10-26T18:22:24Z
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Youbin
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Youbin at 17:34, 26 October 2012
2012-10-26T17:34:13Z
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Youbin
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Youbin at 17:20, 26 October 2012
2012-10-26T17:20:53Z
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FBS at 04:00, 22 October 2012
2012-10-22T04:00:58Z
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FBS
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FBS at 16:06, 19 October 2012
2012-10-19T16:06:35Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <tr><td><b>Figure 10:</b> OD<sub>600</sub> Measurement of culture medium of capsules or beads during cultivation.<a target="_blank" href="https://static.igem.org/mediawiki/2012/<del class="diffchange diffchange-inline">f</del>/<del class="diffchange diffchange-inline">f3</del>/<del class="diffchange diffchange-inline">XMUimmobilization10data</del>.jpg">[click to data]</a></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <tr><td><b>Figure 10:</b> OD<sub>600</sub> Measurement of culture medium of capsules or beads during cultivation.<a target="_blank" href="https://static.igem.org/mediawiki/2012/<ins class="diffchange diffchange-inline">b</ins>/<ins class="diffchange diffchange-inline">bc</ins>/<ins class="diffchange diffchange-inline">CellimmoFigure_10</ins>.jpg">[click to data]</a></div></td></tr>
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FBS
http://2012.igem.org/wiki/index.php?title=Team:XMU-China/cellimmobilization&diff=231206&oldid=prev
FBS at 01:48, 27 September 2012
2012-09-27T01:48:47Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="left"><strong class="subtitle"><a name="_Toc01" id="_Toc01"></a>1. Overview</strong><BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="left"><strong class="subtitle"><a name="_Toc01" id="_Toc01"></a>1. Overview</strong><BR></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>After finishing our circuit's construction, it is time to set up the macroscopic device system. Obviously, the foremost thing we should do is to fix the engineering bacteria from suspended batch cultivation to the tubes of our device, namely cell immobilization. Through detailed <del class="diffchange diffchange-inline">cmparison </del>of three methods in various aspects, we chose the intra-hollow calcium alginate capsule as matrix for our cell immobilization. And the result proves that it is feasible. After that, we started to establish our digital display device and undertaken the continuous cultivation of our engineering bacteria. Meanwhile, cultivation condition and fluorescence intensity of immobilized cells should be made certain.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>After finishing our circuit's construction, it is time to set up the macroscopic device system. Obviously, the foremost thing we should do is to fix the engineering bacteria from suspended batch cultivation to the tubes of our device, namely cell immobilization. Through detailed <ins class="diffchange diffchange-inline">comparison </ins>of three methods in various aspects, we chose the intra-hollow calcium alginate capsule as matrix for our cell immobilization. And the result proves that it is feasible. After that, we started to establish our digital display device and undertaken the continuous cultivation of our engineering bacteria. Meanwhile, cultivation condition and fluorescence intensity of immobilized cells should be made certain.</div></td></tr>
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FBS
http://2012.igem.org/wiki/index.php?title=Team:XMU-China/cellimmobilization&diff=228236&oldid=prev
FBS at 00:53, 27 September 2012
2012-09-27T00:53:04Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 00:53, 27 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><title>XMU-cell immobilization</title></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><title>XMU-cell immobilization</title></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 8:</b> Fluorescence curve of P<sub>BAD</sub>GLT Ca-alginate capsules.<a target="_blank" href="https://static.igem.org/mediawiki/2012/0/02/XMUimmobilization8_11_14_15data.jpg">[click to data]</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 8:</b> Fluorescence curve of P<sub>BAD</sub>GLT Ca-alginate capsules.<a target="_blank" href="https://static.igem.org/mediawiki/2012/0/02/XMUimmobilization8_11_14_15data.jpg">[click to data]</a></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 9:</b> Fluorescence curves of P<sub>BAD</sub>GLT in Ca-alginate beads.<a target="_blank" href="https://static.igem.org/mediawiki/2012/d/dd/XMUimmobilization9data.jpg">[click to data]</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 9:</b> Fluorescence curves of P<sub>BAD</sub>GLT in Ca-alginate beads.<a target="_blank" href="https://static.igem.org/mediawiki/2012/d/dd/XMUimmobilization9data.jpg">[click to data]</a></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="left">If the immobilizing capability of the beads/ intra-hollow capsules is favorable, the bacteria inside rarely leak, and the OD of the medium will be low and steady. We used a microplate reader to measure the OD of the culture medium of the immobilized beads/ intra-hollow capsules. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="left">If the immobilizing capability of the beads/ intra-hollow capsules is favorable, the bacteria inside rarely leak, and the OD of the medium will be low and steady. We used a microplate reader to measure the OD of the culture medium of the immobilized beads/ intra-hollow capsules. </p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 10:</b> OD<sub>600</sub> Measurement of culture medium of capsules or beads during cultivation.<a target="_blank" href="https://static.igem.org/mediawiki/2012/f/f3/XMUimmobilization10data.jpg">[click to data]</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 10:</b> OD<sub>600</sub> Measurement of culture medium of capsules or beads during cultivation.<a target="_blank" href="https://static.igem.org/mediawiki/2012/f/f3/XMUimmobilization10data.jpg">[click to data]</a></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 11:</b> Fluorescence curves of P<sub>BAD</sub>GLT Ca-alginate capsules induced by arabinose after shaking for 3h.<a target="_blank" href="https://static.igem.org/mediawiki/2012/0/02/XMUimmobilization8_11_14_15data.jpg">[click to data]</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 11:</b> Fluorescence curves of P<sub>BAD</sub>GLT Ca-alginate capsules induced by arabinose after shaking for 3h.<a target="_blank" href="https://static.igem.org/mediawiki/2012/0/02/XMUimmobilization8_11_14_15data.jpg">[click to data]</a></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 12:</b> Fluorescence curves of P<sub>BAD</sub>GLT Ca-alginate capsules induced by arabinose after shaking for 10h.<a target="_blank" href="https://static.igem.org/mediawiki/2012/5/56/XMUimmobilization12data.jpg">[click to data]</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 12:</b> Fluorescence curves of P<sub>BAD</sub>GLT Ca-alginate capsules induced by arabinose after shaking for 10h.<a target="_blank" href="https://static.igem.org/mediawiki/2012/5/56/XMUimmobilization12data.jpg">[click to data]</a></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 14:</b> Fluorescence curves of P<sub>BAD</sub>GLT Ca-alginate capsules.<a target="_blank" href="https://static.igem.org/mediawiki/2012/0/02/XMUimmobilization8_11_14_15data.jpg">[click to data]</a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr><td><b>Figure 14:</b> Fluorescence curves of P<sub>BAD</sub>GLT Ca-alginate capsules.<a target="_blank" href="https://static.igem.org/mediawiki/2012/0/02/XMUimmobilization8_11_14_15data.jpg">[click to data]</a></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <td width="<del class="diffchange diffchange-inline">500</del>"><img src="https://static.igem.org/mediawiki/2012/5/56/XMUimmobFigure_15.jpg</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <td width="<ins class="diffchange diffchange-inline">520</ins>"><img src="https://static.igem.org/mediawiki/2012/5/56/XMUimmobFigure_15.jpg</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>" width="<del class="diffchange diffchange-inline">500</del>" align="right" ></td></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>" width="<ins class="diffchange diffchange-inline">520</ins>" align="right" ></td></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </tr></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <tr></div></td></tr>
</table>
FBS