Team:XMU-China

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         <dd class="out">PROGRESS</dd>
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  <div class="tabcon">&nbsp;&nbsp;The aim of this study is to construct a fluorescent digital display device of genetic circuits with synthetic logic gates, which can both display and switch numbers. Similar to electronic circuits, logic regulation operation in cells integrates the extracellular and intracellular signals. We assemble several pairs of promoters and their activators (or repressors) into computing building block of the circuit: PBAD-Arabinose, PcI-CI, and PtetR-TetR. As shown in Figure 1, these computation units act as genetic logic gates perform AND, OR and NOT gates function. In order to light up our digital numbers, we put Green Fluorescent Protein (GFP) in the downstream expression, which is ubiquitous in the field of biological signal protein. Considering that the common types of GFP usually tend to be very stable and hard to "quench", we choose unstable GFP to make our device reusable and convert in a fast speed. This unstable &hellip;
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  <div class="tabcon">
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   <hr><a href="https://2012.igem.org/Template:Team:XMU-China/wetlabjournal">[see more]</a></div>
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  <p>  XMU's 2012 iGEM team has constructed a fluorescent digital display device with synthetic logic gates, which is able to respond to signals by displaying and switching numbers. We put GFP equipped with degradation tags in downstream to illuminate our numbers and change them quickly as well. Considering our engineering background, we accordingly employ cell immobilization to build our device. Engineering bacteria have been embedded in intra-hallow calcium alginate microcapsules and in PDMDAAC-NaCS microcapsules, respectively. In addition, 3D CAD design is performed for a perfect device&#8230;
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   <hr><a href="https://2012.igem.org/Team:XMU-China/abstract">[see more]</a></div>
   
   
   
   
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   <!--PROGRESS-->
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     <div class="tabcon dis">Sunday, August 26th<br>
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     <div class="tabcon dis"><p style="font-size:16px"><strong>Asian iGEM Jamboree, 5-7 October, HKUST</strong></p> <br>
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Digital Display:
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XMU-China has won a GOLD medal and become one of the 17 out of 53 teams advancing to World Championship . We are looking forward to present our project to the whole world at MIT. You are warmly welcomed to attend.<br><br>
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Ligated 18AI and RETPcPb and put the ligation product in 16ºC overnight for the transformation in the next day.<hr>
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<b>Venue: Room 32-123</b><br><br>
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<b>Date: 11:00 AM, Nov 3rd, Saturday</b><br><br>
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Time Delay:
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<hr>
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Goal: To do the ligation of pBAD-RBS0.6 and pBAD-RBS0.07.
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<a href="https://2012.igem.org/Team:XMU-China/wetlabjournal">[see more]</a>
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Isolate plasmids and ran on a gel. The result for analysis was correct. Cause the bands of pBAD hardly to be seem, we increased the volume of pBAD when ligation. <hr>
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Fluorescence Test:
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We changed the abrabinose concentration from high levels to lower ones according to last fluorescence measurements of PBADRLT. Besides, we took some kinetic curves in all concentration levels we had taken before for comparison. The analysis of these data will help us improve our efficiency in the coming fluorescence measurements<hr>
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Design:
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We did some card design and drew some drafts.<hr>
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<a href="https://2012.igem.org/Template:Team:XMU-China/wetlabjournal">[see more]</a>
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         DEVICE DESCRIPTION</p>
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         DEVICE DEMONSTRATION</p>
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       <p style=" font-size:28px; color:#004f7c">E.LUMOLI DISPLAYER</p>
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       <span style=" font-size:28px; color:#004f7c; line-height:24px">E.LUMOLI DISPLAYER</span></p>
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      <div id="description_content">By adding different inducers, cells in tubes can respond to different signals by display and switching numbers.
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      <p style=" font-size:28px; color:#004f7c">&nbsp;</p>
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<td id="title" width="300">Immobilization</td>
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<tr><td id="rb">Synthetic circuits with genetic logic gates, which compute extracellular and intracellular signals and elicit response differently.<a id="more" href="https://2012.igem.org/Team:XMU-China/digitaldisplay">[see more]</a></td>
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<td id="rb">Immobilization of fluorescent <I>E.coli</I> cells. Design a bioreactor for numeric display.<a id="more" href="https://2012.igem.org/Team:XMU-China/cellimmobilization">[see more]</a></td>
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<td><p>Testing the delay of time for GFP expression when we use different strength of RBS.<a id="more" href="https://2012.igem.org/Team:XMU-China/timedelay">[see more]</a></p></td></tr>
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      <td width="300" bgcolor="#f8daef">Synthetic circuits with genetic logic gates, which compute extracellular and intracellular signals and elicit response differently. </td>
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      Immobilization of Fluorescent <I>E.coli</I> cells .Design A Bioreactor for Numeric Display  </td>
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      <td width="300" bgcolor="#f8daef">Testing the delay of time for GFP expression when we use different strength of RBS</td>
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Latest revision as of 03:05, 27 October 2012

XMU



homepage

ABSTRACT
PROGRESS

XMU's 2012 iGEM team has constructed a fluorescent digital display device with synthetic logic gates, which is able to respond to signals by displaying and switching numbers. We put GFP equipped with degradation tags in downstream to illuminate our numbers and change them quickly as well. Considering our engineering background, we accordingly employ cell immobilization to build our device. Engineering bacteria have been embedded in intra-hallow calcium alginate microcapsules and in PDMDAAC-NaCS microcapsules, respectively. In addition, 3D CAD design is performed for a perfect device…

[see more]

Asian iGEM Jamboree, 5-7 October, HKUST


XMU-China has won a GOLD medal and become one of the 17 out of 53 teams advancing to World Championship . We are looking forward to present our project to the whole world at MIT. You are warmly welcomed to attend.

Venue: Room 32-123

Date: 11:00 AM, Nov 3rd, Saturday

[see more]

DEVICE DEMONSTRATION

E.LUMOLI DISPLAYER


By adding different inducers, cells in tubes can respond to different signals by display and switching numbers.

 

Digital Display Immobilization Time delay
Synthetic circuits with genetic logic gates, which compute extracellular and intracellular signals and elicit response differently.[see more] Immobilization of fluorescent E.coli cells. Design a bioreactor for numeric display.[see more]

Testing the delay of time for GFP expression when we use different strength of RBS.[see more]




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