Team:Wisconsin-Madison

From 2012.igem.org

(Difference between revisions)
 
(352 intermediate revisions not shown)
Line 1: Line 1:
-
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
+
{{Template:Wisconsin-Madison}}
-
<head>
+
<html>
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+
-
<meta name="generator" content="MediaWiki 1.16.0" />
+
-
<link rel="shortcut icon" href="/favicon.ico" />
+
-
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2012.igem.org (en)" />
+
-
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:Wisconsin-Madison&amp;action=creativecommons" rel="meta" />
+
-
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
+
-
<link rel="alternate" type="application/atom+xml" title="2012.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" /> <title>Team:Wisconsin-Madison - 2012.igem.org</title>
+
-
<style type="text/css" media="screen, projection">/*<![CDATA[*/
+
-
@import "/wiki/skins/common/shared.css?270";
+
-
@import "/wiki/skins/igem/main.css?270";
+
-
/*]]>*/</style>
+
-
       
+
-
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
+
-
<!--[if lt IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE50Fixes.css?270";</style><![endif]-->
+
-
<!--[if IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE55Fixes.css?270";</style><![endif]-->
+
-
<!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</style><![endif]-->
+
-
<!--[if IE 7]><style type="text/css">@import "/wiki/skins/igem/IE70Fixes.css?270";</style><![endif]-->
+
-
<!--[if lt IE 7]><script type="text/javascript" src="/wiki/skins/common/IEFixes.js?270"></script>
+
-
<meta http-equiv="imagetoolbar" content="no" /><![endif]-->
+
-
+
-
<script>
+
-
var skin="igem",
+
-
stylepath="/wiki/skins",
+
-
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",
+
-
wgArticlePath="/$1",
+
-
wgScriptPath="/wiki",
+
-
wgScriptExtension=".php",
+
-
wgScript="/wiki/index.php",
+
-
wgVariantArticlePath=false,
+
-
wgActionPaths={},
+
-
wgServer="https://2012.igem.org",
+
-
wgCanonicalNamespace="",
+
-
wgCanonicalSpecialPageName=false,
+
-
wgNamespaceNumber=0,
+
-
wgPageName="Team:Wisconsin-Madison",
+
-
wgTitle="Team:Wisconsin-Madison",
+
-
wgAction="view",
+
-
wgArticleId=462,
+
-
wgIsArticle=true,
+
-
wgUserName=null,
+
-
wgUserGroups=null,
+
-
wgUserLanguage="en",
+
-
wgContentLanguage="en",
+
-
wgBreakFrames=false,
+
-
wgCurRevisionId=85423,
+
-
wgVersion="1.16.0",
+
-
wgEnableAPI=true,
+
-
wgEnableWriteAPI=true,
+
-
wgSeparatorTransformTable=["", ""],
+
-
wgDigitTransformTable=["", ""],
+
-
wgMainPageTitle="Main Page",
+
-
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2012.igem.org", "5": "2012.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
+
-
wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2012.igem.org": 4, "2012.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
+
-
wgSiteName="2012.igem.org",
+
-
wgCategories=[],
+
-
wgMWSuggestTemplate="https://2012.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
+
-
wgDBname="2012_igem_org",
+
-
wgSearchNamespaces=[0],
+
-
wgMWSuggestMessages=["with suggestions", "no suggestions"],
+
-
wgRestrictionEdit=[],
+
-
wgRestrictionMove=[];
+
-
</script>               
+
-
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
+
-
<!-- Head Scripts -->
+
-
<script src="/wiki/skins/common/ajax.js?270"></script>
+
-
<script src="/wiki/skins/common/mwsuggest.js?270"></script>
+
-
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
+
-
<!-- jQuery Javascript -->
+
-
<script type='text/javascript'        src ='/common/jquery-latest.min.js'></script>
+
-
<script type='text/javascript'        src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
+
-
        <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' />
+
-
        <link rel='stylesheet' type='text/css' href='/common/table_styles.css' />
+
-
        <link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' />
+
-
<script type='text/javascript'        src ='/forum/forum_scripts.js'></script>
+
-
</head>
+
-
<body              class="mediawiki  ltr ns-0 ns-subject page-Team_Wisconsin-Madison">
 
-
<!-- start content -->
 
-
<p>
 
-
<head>
 
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 
-
<meta name="generator" content="MediaWiki 1.16.0" />
 
-
<link rel="shortcut icon" href="/favicon.ico" />
 
-
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2011.igem.org (en)" />
 
-
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:Wisconsin-Madison&amp;action=creativecommons" rel="meta" />
 
-
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
 
-
<link rel="alternate" type="application/atom+xml" title="2011.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" /> <title>Team:Wisconsin-Madison - 2012.igem.org</title>
 
-
<style type="text/css" media="screen, projection">/*<![CDATA[*/
 
-
@import "/wiki/skins/common/shared.css?270";
 
-
@import "/wiki/skins/igem/main.css?270";
 
-
/*]]>*/</style>
 
-
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
 
-
<!--[if lt IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE50Fixes.css?270";</style><![endif]-->
 
-
<!--[if IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE55Fixes.css?270";</style><![endif]-->
 
-
<!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</style><![endif]-->
 
-
<!--[if IE 7]><style type="text/css">@import "/wiki/skins/igem/IE70Fixes.css?270";</style><![endif]-->
 
-
<!--[if lt IE 7]><script type="text/javascript" src="/wiki/skins/common/IEFixes.js?270"></script>
 
-
<meta http-equiv="imagetoolbar" content="no" /><![endif]-->
 
-
 
-
<script>
 
-
var skin="igem",
 
-
stylepath="/wiki/skins",
 
-
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",
 
-
wgArticlePath="/$1",
 
-
wgScriptPath="/wiki",
 
-
wgScriptExtension=".php",
 
-
wgScript="/wiki/index.php",
 
-
wgVariantArticlePath=false,
 
-
wgActionPaths={},
 
-
wgServer="https://2011.igem.org",
 
-
wgCanonicalNamespace="",
 
-
wgCanonicalSpecialPageName=false,
 
-
wgNamespaceNumber=0,
 
-
wgPageName="Team:Wisconsin-Madison",
 
-
wgTitle="Team:Wisconsin-Madison",
 
-
wgAction="view",
 
-
wgArticleId=954,
 
-
wgIsArticle=true,
 
-
wgUserName=null,
 
-
wgUserGroups=null,
 
-
wgUserLanguage="en",
 
-
wgContentLanguage="en",
 
-
wgBreakFrames=false,
 
-
wgCurRevisionId=196107,
 
-
wgVersion="1.16.0",
 
-
wgEnableAPI=true,
 
-
wgEnableWriteAPI=true,
 
-
wgSeparatorTransformTable=["", ""],
 
-
wgDigitTransformTable=["", ""],
 
-
wgMainPageTitle="Main Page",
 
-
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2011.igem.org", "5": "2011.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
 
-
wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2011.igem.org": 4, "2011.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
 
-
wgSiteName="2011.igem.org",
 
-
wgCategories=[],
 
-
wgMWSuggestTemplate="https://2011.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
 
-
wgDBname="2011_igem_org",
 
-
wgSearchNamespaces=[0],
 
-
wgMWSuggestMessages=["with suggestions", "no suggestions"],
 
-
wgRestrictionEdit=[],
 
-
wgRestrictionMove=[];
 
-
</script>               
 
-
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
 
-
<!-- Head Scripts -->
 
-
<script src="/wiki/skins/common/ajax.js?270"></script>
 
-
<script src="/wiki/skins/common/mwsuggest.js?270"></script>
 
-
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
 
-
<!-- jQuery Javascript -->
 
-
<script type='text/javascript'        src ='/common/jquery-latest.min.js'></script>
 
-
<script type='text/javascript'        src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
 
-
        <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' />
 
-
        <link rel='stylesheet' type='text/css' href='/common/table_styles.css' />
 
-
        <link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' />
 
-
<script type='text/javascript'        src ='/forum/forum_scripts.js'></script>
 
-
</head>
 
-
<body              class="mediawiki  ltr ns-0 ns-subject page-Team_Wisconsin-Madison">
+
<!--end of the navigation menubar!-->
-
  <div id="globalWrapper">
+
-
    <div id="content">
+
-
<a name="top" id="top"></a>
+
-
+
-
<div id="bodyContent">
+
-
+
-
+
-
<!-- start content -->
+
-
<p>
+
-
    <style>
+
-
        hr
+
-
        { border: none;
+
-
            border-top: 1px #000000 solid;
+
-
        }
+
-
 
+
-
        #globalWrapper
+
-
        { //background: url('https://static.igem.org/mediawiki/2011/a/a5/Bg2.jpg') top center;
+
-
            //width: 100%;
+
-
            //position: fixed;
+
-
            //margin-left: auto;
+
-
            //margin-right: auto;
+
-
//margin-top: 0px;
+
-
            background-color: #222423;
+
-
        }
+
-
 
+
-
        #top-section
+
-
        { display: none;
+
-
        }
+
-
 
+
-
        #sddm
+
-
        { margin: 0;
+
-
            padding: 0;
+
-
        z-index: 30
+
-
        }
+
-
 
+
-
        #sddm li
+
-
        { margin: 0;
+
-
            padding: 0;
+
-
            list-style: none;
+
-
            float: left;
+
-
            font: bold 11px arial
+
-
        }
+
-
 
+
-
        #sddm li a
+
-
        { display: block;
+
-
            margin: 0 1px 0 0;
+
-
            padding: 4px 30px;
+
-
            width: 101px;
+
-
            background: #222423;
+
-
            color: #FFF;
+
-
            text-align: center;
+
-
            text-decoration: none
+
-
        }
+
-
 
+
-
        #sddm li a:hover
+
-
        { background: #434343
+
-
        }
+
-
 
+
-
        #sddm div
+
-
        { position: absolute;
+
-
            visibility: hidden;
+
-
            margin: 0;
+
-
            padding: 0;
+
-
            background: #EAEBD8;
+
-
            border: 1px solid #5970B2
+
-
        }
+
-
 
+
-
        #sddm div a
+
-
        { position: relative;
+
-
            display: block;
+
-
            margin: 0;
+
-
            padding: 5px 10px;
+
-
            width: auto;
+
-
            white-space: nowrap;
+
-
            text-align: left;
+
-
            text-decoration: none;
+
-
            background: #eeeeee;
+
-
            color: #535353;
+
-
            font: 11px arial
+
-
        }
+
-
 
+
-
        #sddm div a:hover
+
-
        { background: #4e4e4e;
+
-
            color: #FFF
+
-
        }
+
-
       
+
-
        #footerfake
+
-
        {  position:absolute;
+
-
            bottom: -55px;
+
-
            left:-5px;
+
-
            width:100%;
+
-
            // Height of the footer
+
-
            height:1px;
+
-
            //background:#222423;
+
-
}
+
-
 
+
-
        #fcoli
+
-
        {  position:absolute;
+
-
            bottom: -40px;
+
-
            left:770px;
+
-
            // Height of the footer
+
-
            height:1px;
+
-
            //background:#222423;
+
-
        }
+
-
       
+
-
        table
+
-
        {
+
-
        table-layout: fixed;
+
-
        margin-left: 15px;
+
-
        margin-right: 15px
+
-
        }
+
-
 
+
-
    </style>
+
-
            <!--STYLE ENDS HURRRRRRRRRR-->
+
-
   
+
-
 
+
-
    <head>
+
-
        <script type="text/javascript">
+
-
            var timeout = 500;
+
-
            var closetimer = 0;
+
-
            var ddmenuitem = 0;
+
-
           
+
-
            // open hidden layer
+
-
            function mopen(id)
+
-
            { // cancel close timer
+
-
                mcancelclosetime();
+
-
                // close old layer
+
-
                if(ddmenuitem) ddmenuitem.style.visibility = 'hidden';
+
-
               
+
-
                // get new layer and show it
+
-
                ddmenuitem = document.getElementById(id);
+
-
                ddmenuitem.style.visibility = 'visible';
+
-
            }
+
-
           
+
-
            // close showed layer
+
-
        function mclose()
+
-
            {  if(ddmenuitem) ddmenuitem.style.visibility = 'hidden';
+
-
            }
+
-
           
+
-
            // go close timer
+
-
            function mclosetime()
+
-
            {  closetimer = window.setTimeout(mclose, timeout);
+
-
            }
+
-
 
+
-
            // cancel close timer
+
-
            function mcancelclosetime()
+
-
            {  if(closetimer)
+
-
                {window.clearTimeout(closetimer);
+
-
                closetimer = null;
+
-
                }
+
-
            }
+
-
 
+
-
            // close layer when click-out
+
-
            document.onclick = mclose;
+
-
        </script>
+
-
    </head>
+
-
   
+
-
    <!--BODYYYYYYYYYYYYY STARTS HUR-->
+
-
 
+
-
    <body>
+
-
 
+
-
<center style="position:absolute; left:2px; top:210px">
+
-
<ul id="sddm">
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison" onmouseover="mopen('m1')" onmouseout="mclosetime()">Home</a>
+
-
<div id="m1" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison">Main</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">What is iGEM?</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/ca">Contributions &amp; Attributions</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview" onmouseover="mopen('m2')" onmouseout="mclosetime()">Project</a>
+
-
<div id="m2" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>
+
-
                <a href="https://2011.igem.org/Team:Wisconsin-Madison/directedevolution">Directed Evolution</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
+
-
                <a href="https://2011.igem.org/Team:Wisconsin-Madison/parts">Parts</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/teamoverview" onmouseover="mopen('m3')" onmouseout="mclosetime()">Team</a>
+
-
<div id="m3" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
                <a href="https://2011.igem.org/Team:Wisconsin-Madison/teamoverview">Overview</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teammembers">Members</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamadvisors">Advisors</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamsponsors">Sponsors</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/calender" onmouseover="mopen('m4')" onmouseout="mclosetime()">Notebook</a>
+
-
<div id="m4" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/protocols">Protocols</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/calender">Calendar</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/references">References</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/outreach" onmouseover="mopen('m5')" onmouseout="mclosetime()">Outreach</a>
+
-
<div id="m5" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/scienceolympiad">Science Olympiad</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/reuposterSession">REU Poster Session</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/socialmedia">Social Media</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/safety" onmouseover="mopen('m6')" onmouseout="mclosetime()">Safety</a>
+
-
<div id="m6" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/humanpractice">Human Practice</a>
+
-
</div>
+
-
</li>
+
-
</ul>
+
-
</center>
+
-
+
-
<center>
+
-
    <img src="https://static.igem.org/mediawiki/2011/a/aa/Logo_v2.2.jpg"; style="position:absolute; left:-15px; top:-10px"/>
+
-
</center>
+
-
 
+
-
 
+
-
<p><br> <p><br> <p><br> <p><br> <p><br> <p><br> <p><br> <p><br>
+
-
<!--COOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOONTENT STARTS HUR-->
+
-
 
+
-
 
+
-
<center></center>
+
-
 
+
-
 
+
-
<table border="0" cellspacing="4" cellpadding="0">
+
-
<tr><td>
+
-
 
+
-
<p></td><td align = "left" valign="top" width="150">
+
-
<p><br>
+
 +
  </div>
 +
  <div id = "divthecontent">
 +
    <div id = "divtheoverview" style="height:auto;"><br>
 +
      <p class = "classtheoverview"> <strong> The Translational Coupling Cassette: a tool for evaluating the translation of heterologous proteins in <i>Escherichia coli</i>. </strong></p>
 +
      <p align="left" class = "classtheinlinecontent2"> A powerful method for the production of novel metabolites is the expression of heterologous enzymes in a bacterial host. A common challenge when using non-native genes in metabolic engineering is determining if they are being properly expressed. To address this issue, we have constructed a BioFusion-compatible system for testing the translation of a gene of interest. This system couples the translation of the target gene to a fluorescent reporter gene; fluorescence will only be detected when the target gene is entirely translated. This construct enables synthetic biologists to quickly determine if a gene is being expressed without the need for costly antibodies or analytical instruments (e.g. mass spectrometry). Currently, we are utilizing this cassette to optimize the expression of limonene synthase, an enzyme that catalyzes the production of limonene, a monoterpene with potential as a renewable jet fuel.</p>
 +
    </div><br>
 +
  <div style="float:left; margin-left:20px;"><a href="https://static.igem.org/mediawiki/2012/0/02/IMG_1410.jpg"> <img src="https://static.igem.org/mediawiki/2012/e/e2/UWMIMG_1411.jpeg" width="640" height="427"></a></div>
 +
  <div style="float:right; width: 250px; margin-right: 20px;">
<script src="http://widgets.twimg.com/j/2/widget.js"></script>
<script src="http://widgets.twimg.com/j/2/widget.js"></script>
<script>
<script>
Line 394: Line 22:
   interval: 6000,
   interval: 6000,
   width: 250,
   width: 250,
-
   height: 400,
+
   height: 350,
   theme: {
   theme: {
     shell: {
     shell: {
-
       background: '#878787',
+
       background: '#9c8bb0',
       color: '#ffffff'
       color: '#ffffff'
     },
     },
     tweets: {
     tweets: {
-
       background: '#fafafa',
+
       background: '#d8cee5',
-
       color: '#404040',
+
       color: '#47296d',
-
       links: '#5c64ff'
+
       links: '#a47cd5'
     }
     }
   },
   },
Line 409: Line 37:
     scrollbar: false,
     scrollbar: false,
     loop: false,
     loop: false,
-
     live: false,
+
     live: true,
     hashtags: true,
     hashtags: true,
     timestamp: true,
     timestamp: true,
-
     avatars: false,
+
     avatars: true,
     behavior: 'all'
     behavior: 'all'
   }
   }
Line 418: Line 46:
</script>
</script>
-
</td></table>
 
-
<p><br>
 
-
    <div id="footerfake">
 
-
        <img src="https://static.igem.org/mediawiki/2011/3/3e/Medium_footer.jpg">
 
-
    </div>
 
-
 
-
    <div id="fcoli">
 
-
        <img src="https://static.igem.org/mediawiki/2011/e/ee/Mini_ecoli.png">
 
-
    </div>
 
-
</p>
 
-
<!--
 
-
NewPP limit report
 
-
Preprocessor node count: 4/1000000
 
-
Post-expand include size: 0/2097152 bytes
 
-
Template argument size: 0/2097152 bytes
 
-
Expensive parser function count: 0/100
 
-
-->
 
-
 
-
<!-- Saved in parser cache with key 2011_igem_org:pcache:idhash:954-0!1!0!!en!2!edit=0 and timestamp 20120828013648 -->
 
-
<div class="printfooter"></div>
 
-
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
 
-
<div class="visualClear"></div>
 
-
</div>
 
-
    </div>
 
-
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE  -- RDR  -->
 
-
<div class="visualClear"></div>
 
-
    <div id='footer-box' class='noprint'>
 
-
        <div id="footer">
 
-
              <div id="f-poweredbyico"></div>
 
-
        </div>
 
-
        <div id='footer'> </div> <!-- close footer -->
 
-
    </div> <!-- close footer-box -->
 
-
 
-
<script>if (window.runOnloadHook) runOnloadHook();</script>
 
</div>
</div>
-
<!-- Served in 0.184 secs. --></body>
+
<br>
 +
<br>
 +
<div style=" float:left;">
 +
<p align="left" class="classtheinlinecontent"><strong><span style="font-size:24px"></strong>
 +
</p><br />
 +
<p align="left" class="classtheinlinecontent2"></p>
 +
<br />
 +
<p align="left" class="classtheinlinecontent"><strong><span style="font-size:24px"></strong>
</p>
</p>
-
<!--
+
<br />
-
NewPP limit report
+
<p align="left" class="classtheinlinecontent2">
-
Preprocessor node count: 4/1000000
+
</p><br>
-
Post-expand include size: 0/2097152 bytes
+
<br>
-
Template argument size: 0/2097152 bytes
+
-
Expensive parser function count: 0/100
+
-
-->
+
-
 
+
-
<!-- Saved in parser cache with key 2012_igem_org:pcache:idhash:462-0!1!0!!en!2!edit=0 and timestamp 20120828180435 -->
+
-
 
+
-
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div> <!-- end content -->
+
-
+
-
+
-
<script>if (window.runOnloadHook) runOnloadHook();</script>
+
</div>
</div>
-
<!-- Served in 0.184 secs. --></body>
+
</div>
 +
  <!--div close the content!-->
 +
   
 +
  <div id = "divthelowbanner">
 +
    <img src="http://vtb.bme.wisc.edu/image/temp/iGEMFooter2.png" width="960"></div>
 +
   
 +
  </center>
 +
 
 +
</div> <!--close div container!-->
</html>
</html>

Latest revision as of 20:08, 26 October 2012


The Translational Coupling Cassette: a tool for evaluating the translation of heterologous proteins in Escherichia coli.

A powerful method for the production of novel metabolites is the expression of heterologous enzymes in a bacterial host. A common challenge when using non-native genes in metabolic engineering is determining if they are being properly expressed. To address this issue, we have constructed a BioFusion-compatible system for testing the translation of a gene of interest. This system couples the translation of the target gene to a fluorescent reporter gene; fluorescence will only be detected when the target gene is entirely translated. This construct enables synthetic biologists to quickly determine if a gene is being expressed without the need for costly antibodies or analytical instruments (e.g. mass spectrometry). Currently, we are utilizing this cassette to optimize the expression of limonene synthase, an enzyme that catalyzes the production of limonene, a monoterpene with potential as a renewable jet fuel.