Team:Wisconsin-Madison
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<div id = "divtheoverview"><br> | <div id = "divtheoverview"><br> | ||
- | < | + | <p class = "classtheoverview"> Engineering the limonene production metabolic pathway: TCC. </p> |
- | <p class = "classtheoverview"> | + | <p class = "classtheoverview"> Creating a tool to evaluate the translation of heterologous genes in Eschericia coli. </p> |
</div> | </div> | ||
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<br> | <br> | ||
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+ | <div id = "divthepic"> <img src="http://vtb.bme.wisc.edu/image/temp/limonenePathWay.png" width="400px"></div> | ||
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<div id = "divthedescription" align="left"> | <div id = "divthedescription" align="left"> | ||
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<div style="float:right;clear:right;height:15px;width:185px"></div> | <div style="float:right;clear:right;height:15px;width:185px"></div> | ||
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<!--text wrapper code ends, goes to http://www.csstextwrap.com--> | <!--text wrapper code ends, goes to http://www.csstextwrap.com--> | ||
- | + | <p align="left" class="classtheinlinecontent"> <strong style="font-style:italic; color: rgb(183, 1, 1);">TCC</strong> | |
- | + | </p> | |
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<p align="left" class="classtheinlinecontent"></p> | <p align="left" class="classtheinlinecontent"></p> | ||
+ | <!-- this is no longer neccesary <div id = "divthesmallpic" style="margin-top:-320px"> | ||
</div> | </div> | ||
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<div style="float:left;clear:left;height:15px;width:220px"></div> | <div style="float:left;clear:left;height:15px;width:220px"></div> | ||
<div style="float:right;clear:right;height:15px;width:0px"></div> | <div style="float:right;clear:right;height:15px;width:0px"></div> | ||
- | + | <div style="float:left;clear:left;height:15px;width:220px"><span style="margin-top:-320px"><img src="http://www.google.com/url?source=imgres&ct=img&q=http://www.flyforums.co.uk/customgroupicons/socialgroupicon_22_1275813528.gif&sa=X&ei=bhFBUMapLajbyAG65YDACA&ved=0CAQQ8wc&usg=AFQjCNFU4rKQiDvWJGSicGrK70lp4Hixiw" alt="" width="200" height="150" align="left"></span></div> | |
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<!--text wrapper code ends, goes to http://www.csstextwrap.com--> | <!--text wrapper code ends, goes to http://www.csstextwrap.com--> | ||
- | <p align="right" class="classtheinlinecontent"> <strong style="font-style:italic; color: rgb(183, 1, 1);"></strong></p> | + | <p align="right" class="classtheinlinecontent"> <strong style="font-style:italic; color: rgb(183, 1, 1);">Translational couplding cassette</strong></p> |
- | <p align="left" class="classtheinlinecontent"> | + | <p align="left" class="classtheinlinecontent"> <br> |
+ | In synthetic biology, one of the most powerful tools a research has is the ability to express heterologous genes in Escherichia coli. Unfortunately this does not always work. A common challenge when using non-native genes in metabolic engineering is determining if they are being properly expressed. To address this issue, we have constructed a BioFusion compatible system for diagnosing the failure of a gene expression at the translational level. This system couples the translation of the target gene to a fluorescent reporter gene. Fluorescence will only be detected when the target gene is entirely translated. This construct enables synthetic biologists to quickly determine if a gene is being expressed without the need for costly antibiodies or analytical instruments. | ||
</p> | </p> | ||
- | <p align="center" class="classtheinlinecontent" style="font-style:italic"></p> | + | <p align="center" class="classtheinlinecontent" style="font-style:italic">This tool is based on a phenomenon known as translational coupling. </p> |
</div> | </div> | ||
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- | + | <div id = "divthepic2"> <img src="http://www.diveaqaba.com/news/bullshark/06.jpg" width="400" height="450"></div> | |
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Revision as of 13:18, 2 October 2012
Engineering the limonene production metabolic pathway: TCC.
Creating a tool to evaluate the translation of heterologous genes in Eschericia coli.
TCC
Translational couplding cassette
In synthetic biology, one of the most powerful tools a research has is the ability to express heterologous genes in Escherichia coli. Unfortunately this does not always work. A common challenge when using non-native genes in metabolic engineering is determining if they are being properly expressed. To address this issue, we have constructed a BioFusion compatible system for diagnosing the failure of a gene expression at the translational level. This system couples the translation of the target gene to a fluorescent reporter gene. Fluorescence will only be detected when the target gene is entirely translated. This construct enables synthetic biologists to quickly determine if a gene is being expressed without the need for costly antibiodies or analytical instruments.
This tool is based on a phenomenon known as translational coupling.