Team:Waterloo

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Waterloo’s 2012 iGEM project is a continuation of the 2011 project, In Vivo Protein Fusion Assembly Using Self Excising Ribozymes. This year our hope is to complete the project with the aim of potentially designing future projects which incorporate this system. Self-excising ribozymes are RNA sequences with catalytic properties which allow them to remove themselves and the regions which they flank from an RNA sequence. These are introns; however, with ribozyme self-excision the introns are removed without the aid of protein enzymes. In our project we use self-excising ribozymes to remove an extraneous sequence, an intron, which interrupts the coding sequence of GFP. Upon successful removal of the intron, the two halves of GFP should be ligated together and be able to be translated into a fully functional GFP. By showing that functional fusion proteins can be assembled in-vivo using this system we open up possibilities such as the addition of recombination sites to allow gene shuffling, and regulatory sequences which function at the DNA level but that are removed at the RNA level to create functional proteins.	File:Waterloo logo.png 200px
Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)	File:Waterloo team.png Your team picture
	Team Waterloo

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 |Waterloo’s 2012 iGEM project is a continuation of the 2011 project, In Vivo Protein Fusion Assembly Using Self Excising Ribozymes. This year our hope is to complete the project with the aim of potentially designing future projects which incorporate this system.
-Self-excising ribozymes are RNA sequences with catalytic properties which allow them to remove themselves and regions which they flank from an RNA sequence. These are introns; however, with ribozyme self-excision the introns are removed without the aid of protein enzymes. In our project we use self-excising ribozymes to remove an extraneous sequence, an intron, which interrupts the coding sequence GFP. Upon successful removal of the intron, the two halves of GFP should be ligated together and be able to be translated into a fully functional GFP. By showing functional fusion proteins can be assembled in-vivo using this system we open up possibilities such as the addition of recombination sites DNA level to allow gene shuffling and regulatory sequences which function at the DNA level but removed at the RNA level to create functional proteins.
+Self-excising ribozymes are RNA sequences with catalytic properties which allow them to remove themselves and the regions which they flank from an RNA sequence. These are introns; however, with ribozyme self-excision the introns are removed without the aid of protein enzymes. In our project we use self-excising ribozymes to remove an extraneous sequence, an intron, which interrupts the coding sequence of GFP. Upon successful removal of the intron, the two halves of GFP should be ligated together and be able to be translated into a fully functional GFP. By showing that functional fusion proteins can be assembled in-vivo using this system we open up possibilities such as the addition of recombination sites to allow gene shuffling, and regulatory sequences which function at the DNA level but that are removed at the RNA level to create functional proteins.
 |[[Image:Waterloo_logo.png|200px|right|frame]]