Team:Washington/Protocols/Optogenetics/LightAppExperiments

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Contents

General Protocol for Light App Experiments

Overnight cultures

  1. Cast a gel
  2. Place it in gel box in running buffer
  3. Load samples
  4. Run the gel
  5. Image the gel


96-well plate

Prepare plate

The amount of agarose to use in your gel depends on the DNA in question. Use the following table as a rough guide:


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