Team:Washington/Protocols/Optogenetics/LightAppExperiments

From 2012.igem.org

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==Overnight cultures==
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==Overnight Cultures==
#Cast a gel
#Cast a gel
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==96-well plate==
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==Preparing a 96-Well Plate==
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===Prepare plate===
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==96-Well Plate Culture==
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==Preparing small (~2 in.) plates==
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'''The amount of agarose to use in your gel depends on the DNA in question. Use the following table as a rough guide:'''
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==Making Agar==
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'''← [[Team:Washington/Protocols|Back to Protocols]]'''
'''← [[Team:Washington/Protocols|Back to Protocols]]'''

Revision as of 23:41, 29 September 2012

Contents

General Protocol for Light App Experiments

Overnight Cultures

  1. Cast a gel
  2. Place it in gel box in running buffer
  3. Load samples
  4. Run the gel
  5. Image the gel


Preparing a 96-Well Plate

96-Well Plate Culture

Preparing small (~2 in.) plates

Making Agar

← Back to Protocols

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