Team:Washington/Protocols/EG Assay

From 2012.igem.org

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=Turbidostat: Ethylene Glycol Assay=
=Turbidostat: Ethylene Glycol Assay=
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<li>Created M9 30mM ethylene glycol media</li>
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<ul><li>Sterile 200mL of 5X M9 salts </li>
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<li>2.8mL of filter sterilized 99.99% ethylene glycol</li>
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<li>800mL of sterile double distilled water</li></ul>
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<li>Autoclaved the tubing, syringes, and media vessels</li>

Revision as of 23:27, 3 October 2012


Turbidostat: Ethylene Glycol Assay

  1. Created M9 30mM ethylene glycol media
    • Sterile 200mL of 5X M9 salts
    • 2.8mL of filter sterilized 99.99% ethylene glycol
    • 800mL of sterile double distilled water
  2. Autoclaved the tubing, syringes, and media vessels