Team:Washington/Protocols

From 2012.igem.org

(Difference between revisions)
Line 41: Line 41:
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]
 +
 +
[https://2012.igem.org/Team:Washington/Protocols/Display Yeast Surface Display]
[https://2012.igem.org/Team:Washington/Protocols/Yeast Yeast Transformation]
[https://2012.igem.org/Team:Washington/Protocols/Yeast Yeast Transformation]

Revision as of 01:01, 30 September 2012


General Protocols

General Agarose Gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Colony PCR Protocol

Competent Cell Prep Protocol

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks

Gibson Cloning/Assembly

Isolation of Plasmid DNA (miniprep)

Preparation of Overnight Cultures

Plastic Degradation

[Protocols]

Flu Binders

Kunkel Mutagenesis

Overview of the Kunkel Mutagenesis process

Yeast Surface Display

Yeast Transformation

Optogenetics

Light App Experiments