Team:Washington/Protocols

From 2012.igem.org

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<h1 id='Protocols'>Protocols</h1>
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<h1 id=”General”>General Protocols</h1>
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[http://2012.igem.org/Team:Washington/Protocols/gel_electrophoresis  General Agarose Gel Electrophoresis]
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[http://2012.igem.org/Team:Washington/Protocols/PAGE_gel_electrophoresis General SDS PAGE gel Electrophoresis]
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[http://2012.igem.org/Team:Washington/Protocols/PCR  General PCR Protocol]
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[http://2012.igem.org/Team:Washington/Protocols/Digestion  General Digestion Protocol]
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[http://2012.igem.org/Team:Washington/Protocols/Ligation  General Ligation Protocol]
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[http://2012.igem.org/Team:Washington/Protocols/Transformation  General Transformation Protocol]
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[http://2012.igem.org/Team:Washington/Protocols/Elect. Electroporation (Transformation)]
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[http://2012.igem.org/Team:Washington/Protocols/Colony Colony PCR Protocol]
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[http://2012.igem.org/Team:Washington/Protocols/Competent  Competent Cell Prep Protocol]
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[http://2012.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification]
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[http://2012.igem.org/Team:Washington/Protocols/gene_assembly Gene Assembly With Oligos]
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[http://2012.igem.org/Team:Washington/Protocols/sequencing Sequencing]
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[http://2012.igem.org/Team:Washington/Protocols/CompDesign  Computational Protein Design]
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[http://2012.igem.org/Team:Washington/Protocols/Glycerol_Stocks  Glycerol Stocks]
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[http://2012.igem.org/Team:Washington/Protocols/Gib_Rxn Gibson Cloning/Assembly]
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[http://2012.igem.org/Team:Washington/Protocols/Plas_DNA. Isolation of Plasmid DNA (miniprep)]
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[http://2012.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures]
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[http://2012.igem.org/Team:Washington/Protocols/PCR_Purification PCR Purification]
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[http://2012.igem.org/Team:Washington/Protocols/Gel_Purification Gel Purification]
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<h1 id=”Plastics”>Plastic Degradation</h1>
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[http://2012.igem.org/Team:Washington/Protocols/osmY_Assay osmY Assay]
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[http://2012.igem.org/Team:Washington/Protocols/PUR_Assay PUR Esterase Assay]
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[http://2012.igem.org/Team:Washington/Protocols/EG_Assay Ethylene Glycol Assay]
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<h1 id=”Flu”>Flu Binders</h1>
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[http://2012.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagenesis]
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[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]
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[http://2012.igem.org/Team:Washington/Protocols/Display Yeast Surface Display]
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[http://2012.igem.org/Team:Washington/Protocols/Yeast Yeast Transformation]
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<h1 id=”Optogenetics”>Optogenetics</h1>
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[http://2012.igem.org/Team:Washington/Protocols/Optogenetics/LightAppExperiments Light App Experiments]

Latest revision as of 19:13, 3 October 2012


General Protocols

General Agarose Gel Electrophoresis

General SDS PAGE gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Electroporation (Transformation)

Colony PCR Protocol

Competent Cell Prep Protocol

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks

Gibson Cloning/Assembly

Isolation of Plasmid DNA (miniprep)

Preparation of Overnight Cultures

PCR Purification

Gel Purification

Plastic Degradation

osmY Assay

PUR Esterase Assay

Ethylene Glycol Assay

Flu Binders

Kunkel Mutagenesis

Overview of the Kunkel Mutagenesis process

Yeast Surface Display

Yeast Transformation

Optogenetics

Light App Experiments