Team:Wageningen UR/Journal/week4

From 2012.igem.org

(Difference between revisions)
Line 14: Line 14:
Centrifuged at 15000 RPM, 3 minute
Centrifuged at 15000 RPM, 3 minute
25 ml is dialised, 1st time, overnight.
25 ml is dialised, 1st time, overnight.
 +
 +
 +
 +
 +
22 may
 +
 +
Negative control for SDS
 +
Non induced BL21 CCMV
 +
grew overnight in LB+kan
 +
follow protocol CCMV from day 1, after induction
 +
up to the 4th step of day 2
 +
 +
sample transferred to new tube
 +
stored in iGEM -20 degree Celcius as
 +
BL21 negative - iGEM SDS
 +
 +
 +
24 may
 +
 +
BL21 Plated and greiner tube 10 ml tube
 +
 +
BL21 2 plates freezer
 +
BL21 2 Greiner tubes 30 degree Celcius stove

Revision as of 08:00, 31 May 2012


week 4: 21 may - 25 may

office work

lab work

21 may

Sonified 6 x 30 seconds, output 3-4, amplitude around 20 on ice! cool as much possible, 50 seconds on ice between 30 second steps. lysate in 50 ml disassembly buffer.

Centrifuged at 15000 RPM, 3 minute 25 ml is dialised, 1st time, overnight.



22 may

Negative control for SDS Non induced BL21 CCMV grew overnight in LB+kan follow protocol CCMV from day 1, after induction up to the 4th step of day 2

sample transferred to new tube stored in iGEM -20 degree Celcius as BL21 negative - iGEM SDS


24 may

BL21 Plated and greiner tube 10 ml tube

BL21 2 plates freezer BL21 2 Greiner tubes 30 degree Celcius stove

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