Team:Wageningen UR/Journal/week15

From 2012.igem.org

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== Lab work ==
== Lab work ==
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'''TuYV'''
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* Bricking:
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Last week's minipreps were digested (EcoRI, PstI), the inserts ligated into the chloramphenicol backbone from BBa_J04450 and transformed into our electrocompetent DH5 Alfa ''E. coli''. A PCR check gave negative results, but miniprep was performed anyway.
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* Expression:
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Last week's minipreps were digested (XbaI, Spe1), the inserts ligated into the promotor&RBS-containing backbone from BBa_J04500 and transformed into our electrocompetent BL21 ''E. coli''. Results were unsatisfying st first, so the entire procedure was done again on saturday.

Revision as of 13:40, 18 August 2012

week 15: 6 august - 12 august

Office work

Lab work

TuYV

  • Bricking:

Last week's minipreps were digested (EcoRI, PstI), the inserts ligated into the chloramphenicol backbone from BBa_J04450 and transformed into our electrocompetent DH5 Alfa E. coli. A PCR check gave negative results, but miniprep was performed anyway.

  • Expression:

Last week's minipreps were digested (XbaI, Spe1), the inserts ligated into the promotor&RBS-containing backbone from BBa_J04500 and transformed into our electrocompetent BL21 E. coli. Results were unsatisfying st first, so the entire procedure was done again on saturday.


Written by: Wouter


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