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From 2012.igem.org

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Death system

Outline

We plan to place endonuclease and YhjH gene downstream the xylR induce to make bacteria die and lose their adhesion when expose to xylose.

Description

In order to terminate the overreaction of fatty acid metabolism and cellulose synthesis, we design an elaborate device to avoid it. Firstly, we determine to use endonuclease(BBa_K112106), a molecular which can kill the microbe without inducing cell lysis. To control the expression of endonuclease, a regulator is necessary. We select xylose as a controller because it is more healthy and hard to be absorbed by human. We find an ideal promoter BBa_K625002, which can be activated by the transcriptional regulator XylR. We hope that eating xylose will subsequently induce the expression of endonuclease, ending up with the death of our E.coslim. In addition, we use a pair of genes to strengthen the accuracy of controlling, namely YhjH. YhjH gene will counteract the impact of AdrA. YhjH protein can convert c-di-GMP in to GMP. Thus cells will lose their adhesion and become vulnerable to endonuclease.