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Team:Valencia Biocampus/Protocols - Revision history
2024-03-29T15:58:00Z
Revision history for this page on the wiki
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Lujemomo at 23:24, 26 September 2012
2012-09-26T23:24:56Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">__NOTOC__ </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Valencia_Biocampus/banner}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Valencia_Biocampus/banner}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Valencia_Biocampus/estilo}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Valencia_Biocampus/estilo}}</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__TOC__</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__TOC__</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= '''General Protocols''' =</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= '''General Protocols''' =</div></td></tr>
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Lujemomo
http://2012.igem.org/wiki/index.php?title=Team:Valencia_Biocampus/Protocols&diff=206782&oldid=prev
Lujemomo: /* Mini-prep */
2012-09-26T17:32:01Z
<p><span class="autocomment">Mini-prep</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:32, 26 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Centrifuge 10’ (max. rpm) in order to pellet cell debris and chromosomal DNA.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Centrifuge 10’ (max. rpm) in order to pellet cell debris and chromosomal DNA.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Transfer the supernatant (≈ 800 μL) to the spin column (pipetting to avoid carrying impurities).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Transfer the supernatant (≈ 800 μL) to the spin column (pipetting to avoid carrying impurities).</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <ol><b>Important: DO NOT <del class="diffchange diffchange-inline">TRANSFERING </del>THE PRECIPITATE!</ol></b></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ol><b>Important: DO NOT <ins class="diffchange diffchange-inline">TRANSFER </ins>THE PRECIPITATE!</ol></b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Centrifuge 1’.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Centrifuge 1’.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Flow-though liquid is removed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Flow-though liquid is removed.</div></td></tr>
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Lujemomo
http://2012.igem.org/wiki/index.php?title=Team:Valencia_Biocampus/Protocols&diff=183127&oldid=prev
Lujemomo: /* Yeast Induction protocol */
2012-09-25T23:09:32Z
<p><span class="autocomment">Yeast Induction protocol</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 23:09, 25 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Colonies are picked from petri dish and suspended in suplemented SD media.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Colonies are picked from petri dish and suspended in suplemented SD media.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Incubate overnight</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Incubate overnight</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li>Measure OD.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Resuspend in YPD8%</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Resuspend in YPD8%</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Incubate overnight to 5 OD.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Incubate overnight to 5 OD.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Incubate for 8 to 24 hours</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Incubate for 8 to 24 hours</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Add a final concentration of 0.3 mM of H2O2</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Add a final concentration of 0.3 mM of H2O2</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li>Measure OD and fluorescence.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li>Measure OD and fluorescence <ins class="diffchange diffchange-inline">intensity</ins>.</div></td></tr>
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Lujemomo
http://2012.igem.org/wiki/index.php?title=Team:Valencia_Biocampus/Protocols&diff=143272&oldid=prev
Lujemomo: /* Mini-prep */
2012-09-22T20:43:34Z
<p><span class="autocomment">Mini-prep</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Add 250 μL of Lysis Solution.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Add 250 μL of Lysis Solution.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Mix it inverting the tube 4-6 times <b>(DO NOT VORTEX!)</b> until solution gets viscous and slightly clear. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Mix it inverting the tube 4-6 times <b>(DO NOT VORTEX!)</b> until solution gets viscous and slightly clear. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <ol>Important: Do not incubate more than 5 min.</ol></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ol<ins class="diffchange diffchange-inline">><b</ins>>Important: Do not incubate more than 5 min.<ins class="diffchange diffchange-inline"></b></ins></ol></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Add 350 μL of Neutralization Solution.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Add 350 μL of Neutralization Solution.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Mix it inverting the tube 4-6 times. Incubate in ice for 15-30 min.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Mix it inverting the tube 4-6 times. Incubate in ice for 15-30 min.</div></td></tr>
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Lujemomo
http://2012.igem.org/wiki/index.php?title=Team:Valencia_Biocampus/Protocols&diff=143269&oldid=prev
Lujemomo at 20:41, 22 September 2012
2012-09-22T20:41:19Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><td>DNA linearized plasmid Backbone (25 ng/uL)</td></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><td>DNA linearized plasmid Backbone (25 ng/uL)</td></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><td>Plasmid DNA</td></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><td>Plasmid DNA</td></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><td><b>LiAc 1M</b></td></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><td><b>LiAc 1M</b></td></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><td><b>LiAc 1M</b></td></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><td><b>LiAc 1M</b></td></div></td></tr>
</table>
Lujemomo
http://2012.igem.org/wiki/index.php?title=Team:Valencia_Biocampus/Protocols&diff=142855&oldid=prev
Lujemomo at 18:34, 22 September 2012
2012-09-22T18:34:05Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ol><p style="font-size:x-large;">Protocols</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ol><p style="font-size:x-large;">Protocols</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>------------</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>------------</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__TOC__</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__TOC__</div></td></tr>
</table>
Lujemomo
http://2012.igem.org/wiki/index.php?title=Team:Valencia_Biocampus/Protocols&diff=142853&oldid=prev
Lujemomo at 18:33, 22 September 2012
2012-09-22T18:33:38Z
<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="font-size:x-large;">Protocols</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><ol></ins><p style="font-size:x-large;">Protocols</p></div></td></tr>
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Lujemomo
http://2012.igem.org/wiki/index.php?title=Team:Valencia_Biocampus/Protocols&diff=142850&oldid=prev
Lujemomo at 18:33, 22 September 2012
2012-09-22T18:33:18Z
<p></p>
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Lujemomo
http://2012.igem.org/wiki/index.php?title=Team:Valencia_Biocampus/Protocols&diff=142846&oldid=prev
Lujemomo at 18:33, 22 September 2012
2012-09-22T18:33:00Z
<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 18:33, 22 September 2012</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'''PROTOCOLS'''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p style="font-size:xx-large;">Protocols</p</ins></div></td></tr>
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Lujemomo
http://2012.igem.org/wiki/index.php?title=Team:Valencia_Biocampus/Protocols&diff=142837&oldid=prev
Lujemomo at 18:31, 22 September 2012
2012-09-22T18:31:44Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Valencia_Biocampus/menu2}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Valencia_Biocampus/menu2}}</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''PROTOCOLS'''</del></div></td><td colspan="2"> </td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''PROTOCOLS'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">------------</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__TOC__</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__TOC__</div></td></tr>
</table>
Lujemomo