Team:Valencia Biocampus

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(Talking Life)
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== Talking Life ==
 
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">The Team</span>
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<span class="sdt_descr">Know the team</span>
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</span>
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</a>
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<div class="sdt_box">
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      <!-- <a href="https://2012.igem.org/Team:Valencia_Biocampus/Team">The Team</a>-->
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      <!-- <a href="https://2012.igem.org/Team:Valencia_Biocampus/Team2"> Student Members </a> -->
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                                                        <a href="https://2012.igem.org/Team:Valencia_Biocampus/c3po">Student Members </a>
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                                                        <a href="https://2012.igem.org/Team:Valencia_Biocampus/c3poAdvisors"> Advisors </a>
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                                                        <a href="https://2012.igem.org/Team:Valencia_Biocampus/c3poInstructors"> Instructors </a>
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<a href="https://2012.igem.org/Team:Valencia_Biocampus/Universities">The Universities</a>
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'''Talking life in 150 words'''
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<!--<a href="https://2012.igem.org/Team:Valencia_Biocampus/Akw">Aknowledgements</a>-->
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                                                        <a href="https://2012.igem.org/Team:Valencia_Biocampus/Akw2">Aknowledgements</a>
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                                                        <a href="https://2012.igem.org/Team:Valencia_Biocampus/Attributions">Attributions</a>
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''Do you speak to your bacteria? We do. We have designed, constructed and characterized an inter-specific translator based on light pulses that allows to literally dialogue with microorganisms. We have built seven biobricks with fluorescent proteins under the control of environmentally-sensitive promoters. The process is as follows: human voice messages are electronically- and then light-encoded in excitation wavelengths, and microbial proteins’ emission wavelengths are electronically- and voice-encoded back. We have used this system to find out the fermentative status of budding yeast and to dialogue with ''E. coli'' allowing it to answer questions such as “are you hungry?” The three pillars of our project (human practices, modeling and wetlab) yielded continuous feedback with each other, illustrating an integrated interdisciplinary approach. For example, in human practices, we qualitatively discussed the possibility of cheater mutant (“liers”), which was quantitatively supported by our results in both our modeling simulations and in the wetlab.''
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                                                  <!--     <a href="https://2012.igem.org/Team:Valencia_Biocampus/Gallery">Gallery</a>    -->
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                                                  <!--   <a href="https://2012.igem.org/Team:Valencia_Biocampus/galeria">Gallery</a> -->
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                                                        <a href="https://2012.igem.org/Team:Valencia_Biocampus/galeria2">Gallery</a>
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                                                    <!--    <a href="https://2012.igem.org/Team:Valencia_Biocampus/contactUs">Contact Us</a> -->
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                                                        <a href="https://2012.igem.org/Team:Valencia_Biocampus/contactUs2">Contact Us</a>
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</div>
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</li>
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<li>
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<a href="#">
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<img src="https://static.igem.org/mediawiki/2012/1/1a/C3po_valencia.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">The Project</span>
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<span class="sdt_descr">Where ideas get born</span>
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</span>
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</a>
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<div class="sdt_box">
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<a href="https://2012.igem.org/Team:Valencia_Biocampus/Project">Overview</a>
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'''Who said scientists don't care about microbial cultures?'''
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<!-- <a href="https://2012.igem.org/Team:Valencia_Biocampus/Bacterium">Bacteria</a> -->
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<a href="https://2012.igem.org/Team:Valencia_Biocampus/Bacterium2">Bacteria</a>
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This year, València Biocampus team members are going to break down communication frontiers among species through an artificial language based on wavelengths, which will allow us to know more about the requirements of the microorganisms we thrive everyday in our labs. Depending on different metabolic conditions, our microorganisms (bacteria and yeast) will express genes that are under the control of promoters sensitive to these nutrients and codify for different fluorescent proteins. Therefore, by using a fluorimeter controlled by voice-recognition software, we are able to ask and get answers (like real-life talking!) about the bacteria environment! Moreover we have a brand-new experience, related to UV-regulated promoter, in which we dictate what the bacterium has to produce!
 
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We have achieved good results so far. After ordering a total number of 7 different constructions (5 for bacteria, 2 for yeast) synthesized chemically we transformed competent cells and they responded very well to their medium conditions. We are, right now, working on improving the nutrient-sensing part.
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<!-- <a href="https://2012.igem.org/Team:Valencia_Biocampus/Yeast">Yeast</a> -->
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                                                        <a href="https://2012.igem.org/Team:Valencia_Biocampus/Yeast2">Yeast</a>
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Our final goal is to characterize and model completely well bacteria interaction regarding to these nutrient factors, how they behave then and how they answer to the different conditions. In addition, we would like to design a self-made-but-cool device for carrying out the whole trial. And last, but not least, our Human Practices team has an awesome story-telling script for the audience!
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                                                        <a href="https://2012.igem.org/Team:Valencia_Biocampus/Modeling">Modeling</a>
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                                                        <a href="https://2012.igem.org/Team:Valencia_Biocampus/talking">Talking Interfaces</a>
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                                                        <a href="https://2012.igem.org/Team:Valencia_Biocampus/Notebook">Notebook</a>
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</div>
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</li>
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<li>
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<a href="#">
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<img src="https://static.igem.org/mediawiki/2012/b/b4/Biobrick_valencia.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Biobricks</span>
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<span class="sdt_descr">The parts we made</span>
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</span>
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</a>
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<div class="sdt_box">
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<a href="https://2012.igem.org/Team:Valencia_Biocampus/Biobricks">Submitted biobricks</a>
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<a href="https://2012.igem.org/Team:Valencia_Biocampus/Making_our_Biobricks">Making our Biobricks</a>
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<!-- <a href="https://2012.igem.org/Team:Valencia_Biocampus/Notebook">Notebook</a> -->
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</div>
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</li>
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<li>
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<a href="#">
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<img src="https://static.igem.org/mediawiki/2012/7/70/Modeling_menu_vlc.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Modeling</span>
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<span class="sdt_descr">Inside knowlegde</span>
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</span>
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</a>
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                                        <div class="sdt_box">
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<a href="https://2012.igem.org/Team:Valencia_Biocampus/Modeling">Characterizing Biobricks</a>
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<a href="https://2012.igem.org/Team:Valencia_Biocampus/modhp">for Human Practices</a>
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We are looking forward to seeing you all and enjoy together this amazing experience!
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</div>
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</li>
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<li>
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<a href="#">
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<img src="https://static.igem.org/mediawiki/2012/9/9d/Human_valencia.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Human Practices</span>
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<span class="sdt_descr">Talking life</span>
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</span>
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</a>
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                                        <div class="sdt_box">
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<a href="https://2012.igem.org/Team:Valencia_Biocampus/Ethics2">Ethical issues</a>
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<!-- <a href="https://2012.igem.org/Team:Valencia_Biocampus/Ethics">Ethical issues</a> -->
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== Crowdfunding ==
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<a href="https://2012.igem.org/Team:Valencia_Biocampus/Safety">Safety</a>
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</div>
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</li>
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<li>
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                                        <a href="https://2012.igem.org/Team:Valencia_Biocampus/Achievements">
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<img src="https://static.igem.org/mediawiki/2012/e/e6/Albaachievements8.png" alt=""/>
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<span class="sdt_active"></span>
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<span class="sdt_wrap">
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<span class="sdt_link">Achievements</span>
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<span class="sdt_descr">What we did</span>
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</span>
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</a>
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</li>
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</ul>
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</div>
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More information: http://www.indiegogo.com/vlcbiocampusIGEM
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*
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 +
* conditions and the following disclaimer.
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 +
* of conditions and the following disclaimer in the documentation and/or other materials
 +
* provided with the distribution.
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              * or promote products derived from this software without specific prior written permission.
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              * THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
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              * MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
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              *  COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
 +
              *  EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE
 +
              *  GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED
 +
              * AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING
 +
              *  NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED
 +
              * OF THE POSSIBILITY OF SUCH DAMAGE.
 +
              *
 +
              */
 +
        </script>
 +
       
 +
        <script type="text/javascript">
 +
            $(function() {
 +
/**
 +
* for each menu element, on mouseenter,
 +
* we enlarge the image, and show both sdt_active span and
 +
* sdt_wrap span. If the element has a sub menu (sdt_box),
 +
* then we slide it - if the element is the last one in the menu
 +
* we slide it to the left, otherwise to the right
 +
*/
 +
                $('#sdt_menu > li').bind('mouseenter',function(){
 +
var $elem = $(this);
 +
$elem.find('img')
 +
.stop(true)
 +
.animate({
 +
'width':'160px',
 +
'height':'160px',
 +
'left':'0px'
 +
},400,'easeOutBack')
 +
.andSelf()
 +
.find('.sdt_wrap')
 +
    .stop(true)
 +
.animate({'top':'140px'},500,'easeOutBack')
 +
.andSelf()
 +
.find('.sdt_active')
 +
    .stop(true)
 +
.animate({'height':'160px'},300,function(){
 +
var $sub_menu = $elem.find('.sdt_box');
 +
if($sub_menu.length){
 +
var left = '160px';
 +
if($elem.parent().children().length == $elem.index()+1)
 +
left = '-160px';
 +
$sub_menu.show().animate({'left':left},200);
 +
}
 +
});
 +
}).bind('mouseleave',function(){
 +
var $elem = $(this);
 +
var $sub_menu = $elem.find('.sdt_box');
 +
if($sub_menu.length)
 +
$sub_menu.hide().css('left','0px');
 +
 +
$elem.find('.sdt_active')
 +
.stop(true)
 +
.animate({'height':'0px'},300)
 +
.andSelf().find('img')
 +
.stop(true)
 +
.animate({
 +
'width':'0px',
 +
'height':'0px',
 +
'left':'85px'},400)
 +
.andSelf()
 +
.find('.sdt_wrap')
 +
.stop(true)
 +
.animate({'top':'25px'},500);
 +
});
 +
            });
 +
        </script>
-
<!-- %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% -->
+
        <style>
-
<!-- %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% -->
+
body{ color:#CCCCCC}
-
<!-- %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% -->
+
-
<!-- SECCIÓN DERECHA DE LA PAGINA -->
+
ul.sdt_menu{
 +
margin:0;
 +
padding:0;
 +
list-style: none;
 +
font-family:"Myriad Pro", "Trebuchet MS", sans-serif;
 +
font-size:14px;
 +
width:1020px;
 +
       
 +
}
 +
ul.sdt_menu a{
 +
text-decoration:none;
 +
outline:none;
 +
}
 +
ul.sdt_menu li{
 +
float:left;
 +
width:160px;
 +
height:85px;
 +
position:relative;
 +
cursor:pointer;
 +
}
 +
ul.sdt_menu li > a{
 +
position:absolute;
 +
top:0px;
 +
left:0px;
 +
width:160px;
 +
height:85px;
 +
z-index:12000;
 +
background:transparent url(https://static.igem.org/mediawiki/2012/5/5c/Overlay.png) no-repeat bottom right;
 +
-moz-box-shadow:0px 0px 2px #000 inset;
 +
-webkit-box-shadow:0px 0px 2px #000 inset;
 +
box-shadow:0px 0px 2px #000 inset;
 +
}
 +
ul.sdt_menu li a img{
 +
border:none;
 +
position:absolute;
 +
width:0px;
 +
height:0px;
 +
bottom:0px;
 +
left:85px;
 +
z-index:100000;
 +
-moz-box-shadow:0px 0px 4px #000;
 +
-webkit-box-shadow:0px 0px 4px #000;
 +
box-shadow:0px 0px 4px #000;
 +
}
 +
ul.sdt_menu li span.sdt_wrap{
 +
position:absolute;
 +
top:25px;
 +
left:0px;
 +
width:160px;
 +
height:60px;
 +
z-index:15000;
 +
}
 +
ul.sdt_menu li span.sdt_active{
 +
position:absolute;
 +
background:#111;
 +
top:85px;
 +
width:160px;
 +
height:0px;
 +
left:0px;
 +
z-index:14000;
 +
-moz-box-shadow:0px 0px 4px #000 inset;
 +
-webkit-box-shadow:0px 0px 4px #000 inset;
 +
box-shadow:0px 0px 4px #000 inset;
 +
}
 +
ul.sdt_menu li span span.sdt_link,
 +
ul.sdt_menu li span span.sdt_descr,
 +
ul.sdt_menu li div.sdt_box a{
 +
margin-left:15px;
 +
text-transform:uppercase;
 +
text-shadow:1px 1px 1px #000;
 +
}
 +
ul.sdt_menu li span span.sdt_link{
 +
color:#fff;
 +
font-size:20px;
 +
float:left;
 +
clear:both;
 +
}
 +
ul.sdt_menu li span span.sdt_descr{
 +
color:#0B75AF;
 +
float:left;
 +
clear:both;
 +
width:150px;                                            /*For dumbass IE7*/
 +
font-size:10px;
 +
letter-spacing:1px;
 +
}
 +
ul.sdt_menu li div.sdt_box{
 +
display:block;
 +
position:absolute;
 +
width:200px;
 +
overflow:hidden;
 +
height:270px;
 +
top:85px;
 +
left:0px;
 +
display:none;
 +
background:#000;
 +
}
 +
ul.sdt_menu li div.sdt_box a{
 +
float:left;
 +
clear:both;
 +
line-height:30px;
 +
color:#0B75AF;
 +
}
 +
ul.sdt_menu li div.sdt_box a:first-child{
 +
margin-top:15px;
 +
}
 +
ul.sdt_menu li div.sdt_box a:hover{
 +
color:#fff;
 +
}
-
<div id="HomeRight">
 
-
<!-- CUENTA ATRÁS -->
+
/* body{
 +
background:#333 url(https://static.igem.org/mediawiki/2012/f/f9/Fondo_gris.jpeg) repeat top left;                            /* fondo de pagina */
 +
font-family:Arial;
 +
}*/
 +
span.reference{
 +
position:fixed;
 +
left:10px;
 +
bottom:10px;
 +
font-size:12px;
 +
}
 +
span.reference a{
 +
color:#aaa;
 +
text-transform:uppercase;
 +
text-decoration:none;
 +
text-shadow:1px 1px 1px #000;
 +
margin-right:30px;
 +
}
 +
span.reference a:hover{
 +
color:#ddd;
 +
}
 +
ul.sdt_menu{
 +
margin-top:15px;
 +
}
 +
h1.title{
 +
text-indent:-9000px;
 +
background:transparent url(title.png) no-repeat top left;
 +
width:633px;
 +
height:69px;
 +
}
-
<html>
 
-
    Time goes by...
 
-
    <div align="center"; style="background:#000;width:240px; height:240px;font:0px sans-serif;text-align:left;">
 
-
    <object classid="clsid:d27cdb6e-ae6d-11cf-96b8-444553540000" id="cdtw" width="240" height="240" style="outline:none">
 
-
        <param name="movie" value="http://cdn.countingdownto.com/c/w.swf" />
 
-
        <param name="flashvars" value="eid=108788" />
 
-
        <param name="allowscriptaccess" value="always" />
 
-
        <param name="bgcolor" value="#000000" />
 
-
        <embed name="cdtw" src="http://cdn.countingdownto.com/c/w.swf" flashvars="eid=108788" type="application/x-shockwave-flash" width="240" height="240" 
 
-
          allowscriptaccess="always" bgcolor="#000000" style="outline:none">
 
-
        </embed>
 
-
    </object>
 
-
    <br/>
 
-
    </div>
 
-
    <br><br>
 
-
</html>
 
-
<!-- %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% -->
+
</style>
-
<!-- %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% -->
+
-
 
+
-
<!--- TWITTER --->
+
-
<div style="float: left; width: 270px;">
+
-
<h1>Follow us on Twiter</h1>
+
-
 
+
-
<html>
+
-
    <script src="http://widgets.twimg.com/j/2/widget.js"></script>
+
-
    <script>
+
-
        new TWTR.Widget({
+
-
            version: 2,
+
-
            type: 'profile',
+
-
            rpp: 4,
+
-
            interval: 6000,
+
-
            width: 270,
+
-
            height: 200,
+
-
            theme:{
+
-
                shell: {
+
-
                    background: '#333333',
+
-
                    color: '#ffffff'
+
-
                },
+
-
                tweets: {
+
-
                    background: '#000000',
+
-
                    color: '#ffffff',
+
-
                    links: '#3399ff'
+
-
                }
+
-
            },
+
-
            features: {
+
-
                scrollbar: false,
+
-
                loop: true,
+
-
                live: true,
+
-
                hashtags: true,
+
-
                timestamp: true,
+
-
                avatars: false,
+
-
                behavior: 'all'
+
-
            }
+
-
        }).render().setUser('vlc_biocampus').start();
+
-
</script>
+
</html>
</html>
-
 
-
 
-
<!-- %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% -->
 
-
<!-- %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% -->
 
-
 
-
<!--- FACEBOOK --->
 
-
<html>   
 
-
    <!-- FACEBOOK: crear conexion. Necesario para la parte derecha -->
 
-
    <div id="fb-root"></div>
 
-
        <script>(function(d, s, id) {
 
-
            var js, fjs = d.getElementsByTagName(s)[0];
 
-
            if (d.getElementById(id)) return;
 
-
            js = d.createElement(s); js.id = id;
 
-
            js.src = "//connect.facebook.net/en_US/all.js#xfbml=1";
 
-
            fjs.parentNode.insertBefore(js, fjs);
 
-
            }(document, 'script', 'facebook-jssdk'));
 
-
        </script> 
 
-
 
-
        <!-- FACEBOOK: para dejar comentarios directamente -->
 
-
        <!--
 
-
        <h1>Follow us on Facebook</h1>
 
-
        <p>Be the first one!!</p>
 
-
        <div class="fb-comments" data-href="https://www.facebook.com/pages/Val%C3%A8ncia-Biocampus-iGEM-2012/284330661673814" data-num-posts="6"
 
-
                data-width="470">
 
-
        </div>
 
-
        -->
 
-
 
-
        <!-- FACEBOOK: caja -->
 
-
        <div class="fb-like-box" data-href="https://www.facebook.com/pages/Val%C3%A8ncia-Biocampus-iGEM-2012/284330661673814" data-width="270"
 
-
                data-show-faces="true" data-stream="true" data-header="true">
 
-
        </div>
 
-
</html>
 
-
 
-
</div>    <!-- FIN DE ESTILO DEFINIDO-->
 
-
 
-
 
-
<!-- %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% -->
 
-
<!-- %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% -->
 
-
<!-- 3 ICONOS PARA SEGUIR: FACEBOOK, TWITTER, TUENTI -->
 
-
 
-
<!-- Follow us:
 
-
<br>
 
-
<img name="valencia_follow_us" src="https://static.igem.org/mediawiki/2010/9/9e/Valencia_follow_us_t.png" width="240" height="61" border="0" id="valencia_follow_us" usemap="#m_valencia_follow_us" alt="" /><map name="m_valencia_follow_us" id="m_valencia_follow_us">
 
-
<area shape="rect" coords="150,0,209,61" href="twenti" target="_blank" alt="Valencia iGEM Team Facebook" />
 
-
<area shape="rect" coords="31,0,90,61" href="https://www.facebook.com/pages/Val%C3%A8ncia-Biocampus-iGEM-2012/284330661673814" target="_blank" alt="" />
 
-
<area shape="rect" coords="91,0,150,61" href="http://twitter.com/vlc_biocampus" target="_blank" alt="Valencia Biocampus iGEM Team Twitter" />
 
-
</map>
 
-
-->
 
-
 
-
<!-- %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% -->
 
-
<!-- %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% -->
 
-
 
-
<br>
 
-
</div>
 

Revision as of 10:49, 14 September 2012