Team:Valencia/compo liquid

From 2012.igem.org



Compo medium


Reagents and Materials

  • 1’125 μl COMPO/mL distilled water


Protocol

  1. Pick up with a micropipette 1’125 μl COMPO in 25mL volume for each plate.
  2. Set pH at 7’4.
  3. Autoclave at 121ºC during 20 minutes.
  4. Fill COMPO plus agar in the plates and leave until solidify.
  5. Optative: For doing solid medium used 3% agar.