Team:Valencia/achivements

From 2012.igem.org

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<li>Designed the first <b>photosynthetically powered biolamp</b>, an environmentally friendly lamp that uses light as energy source and living cells as light emissor.</li><br>
<li>Designed the first <b>photosynthetically powered biolamp</b>, an environmentally friendly lamp that uses light as energy source and living cells as light emissor.</li><br>
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<li>'''Developed an integrative [https://2009.igem.org/Team:Valencia/Hardware experimental system]''' to control the LECs, which is able to '''apply a precise voltage (between 0V-24V) during a precise period of time (with intervals as small as 20 ms.)''' controlled by a computer. The [https://2009.igem.org/Team:Valencia/Hardware experimental device] was integrated with a continuous luminometer in order to measure the light emission as a function of these parameters.<br></li><br>
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<li>Applied and introduced for the very first time on the iGEM contest the <b>synthetic ecology</b> concept, designing an <b>artificial consortium</b> between two marine organisms. (link a overview).<br></li><br>
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<li>[https://2009.igem.org/Team:Valencia/WetLab/YeastTeam/Results '''Precisely characterized the LEC'''], studying the factors that can be tuned (like voltage and shock time) and proving in a [https://2009.igem.org/Team:Valencia/Parts/Characterization wide range of experiments] that '''aequorin-engineered yeast can be stimulated with electric current'''. From these works we have established that '''the refreshing time of our LEC is of about 10 seconds''' placing lots of importance on [http://partsregistry.org/wiki/index.php?title=Part:BBa_K222000 our thoroughfully detailed BioBrick] regarding future works.</li><br><br>
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<li>Built a <b>theoretical model</b> for the processes involved in our biolamp in order to predict and optimize the conditions where our biolamp works more optimal (link a modeling).</li><br><br>
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<li>Built [https://2009.igem.org/Team:Valencia/OurModel '''a model] to describe the calcium dynamics in yeast''', and its response to electrical stimuli. The model demonstrate its capability to '''describe the performed experiments and and allows [https://2009.igem.org/Team:Valencia/Simulations the determination of critical parameters'''] of the process like membrane impendancy or conductance. Additionally, [https://2009.igem.org/Team:Valencia/StochasticApproach '''a stochastic model'''] was developed in order to study posible aleatory process that could have an influence in the performance of the system.<br><br></li>
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<li>Transformed the PCC7942 <i>Synechococcus elongatum</i> strain in order to test and characterize the psbAI promoter (link a genetic engineering).<br><br></li>
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<li>Developed '''an impressive [https://2009.igem.org/Team:Valencia/Human Human Practices] part''' of the project. Resulting in the publication of the book [https://2009.igem.org/Team:Valencia/Human '''Sins, Ethics and Biology''']. Result of the review of more than 50 Human Practices articles and the interview to experts.<br></li><br>
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<li><b>Submitted a well characterized part</b> as a BioBrick: <b>the <i>Syenchococcus elongatus</i> psbAI promoter (BBA_754000)</b> and placed it on the backbone plasmid pSB1C3 (link al biobrick/o a submitted parts, lo que mejor esté)<br></li><br>
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<li>Achieved '''[https://2009.igem.org/Team:Valencia/Human the largest survey] ever made on Synthetic Biology''', which is a good occasion to thank each single contributor to the survey, specially those who described '''[https://2009.igem.org/Team:Valencia/Definitions their definition of Synthetic Biology]'''.<br><br></li>
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<li> <b>Opened a bilingual (English and Spanish) weblog about synthetic biology,</b> focusing specially on the divulgation and human practices issues; being even mentioned on the iGEM publicity page  (link a blog) .<br><br></li>
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<li>Helped TUDelft and NTU-Singapore whith their respectives surveys, as well as helped Paris Team answering their questions about their iPhone application.<br><br></li>
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<li><b>Established collaborations </b> with the iGEM teams from the University of Copenhagen,Chile Catholic Unviersity, Uppsala and University College of London (link a collaborations).<br><br></li>
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<li>Tested and characterized new culture medium for growing cyanobacteria in our lab conditions (link a cultures).<br><br></li>
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<li><b>Transformed a DH5α <i>E. coli</i> strain</b> for producing AHL in order <b>to test <i>Allivibrio fischeri</i> bioluminescence<b> (link a genetic engineering).<br><br></li>
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<Li><b>Fulfilled all the Bronze, Silver, and Gold Medal requirements<b> requested by the constest (having fullfied in the last case two of the three purposed requierements)(link a judging criteria).<br><br></li>
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Revision as of 22:01, 26 September 2012



Achievements

We have:

  1. Designed the first photosynthetically powered biolamp, an environmentally friendly lamp that uses light as energy source and living cells as light emissor.

  2. Applied and introduced for the very first time on the iGEM contest the synthetic ecology concept, designing an artificial consortium between two marine organisms. (link a overview).

  3. Built a theoretical model for the processes involved in our biolamp in order to predict and optimize the conditions where our biolamp works more optimal (link a modeling).


  4. Transformed the PCC7942 Synechococcus elongatum strain in order to test and characterize the psbAI promoter (link a genetic engineering).

  5. Submitted a well characterized part as a BioBrick: the Syenchococcus elongatus psbAI promoter (BBA_754000) and placed it on the backbone plasmid pSB1C3 (link al biobrick/o a submitted parts, lo que mejor esté)

  6. Opened a bilingual (English and Spanish) weblog about synthetic biology, focusing specially on the divulgation and human practices issues; being even mentioned on the iGEM publicity page (link a blog) .

  7. Established collaborations with the iGEM teams from the University of Copenhagen,Chile Catholic Unviersity, Uppsala and University College of London (link a collaborations).

  8. Tested and characterized new culture medium for growing cyanobacteria in our lab conditions (link a cultures).

  9. Transformed a DH5α E. coli strain for producing AHL in order to test Allivibrio fischeri bioluminescence (link a genetic engineering).

  10. Fulfilled all the Bronze, Silver, and Gold Medal requirements requested by the constest (having fullfied in the last case two of the three purposed requierements)(link a judging criteria).