http://2012.igem.org/wiki/index.php?title=Team:Valencia/BG-11HGs&feed=atom&action=historyTeam:Valencia/BG-11HGs - Revision history2024-03-29T01:39:44ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Valencia/BG-11HGs&diff=235087&oldid=prevDaie07: Created page with "{{:Team:Valencia/menu}} {{:Team:Valencia/fondoweb}} <html> <div id="Titulos"> <br><br> BG-11 for Harvard/Golden in medium solid. </div> <div id="HomeCenterCenter"><p align="ju..."2012-09-27T03:03:32Z<p>Created page with "{{:Team:Valencia/menu}} {{:Team:Valencia/fondoweb}} <html> <div id="Titulos"> <br><br> BG-11 for Harvard/Golden in medium solid. </div> <div id="HomeCenterCenter"><p align="ju..."</p>
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BG-11 for Harvard/Golden in medium solid.<br />
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<u><h4>Reagents and Materials</h4></u><br />
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<li>100ml BG-11 Harvard/Golden liquid</li><br />
<li>Antibiotics.</li><br />
<li>3% Agar.</li><br />
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<u><h4>Protocol</u></h4><br />
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<li>Add 100ml of BG-11 Harvard/Golden liquid in a Erlenmeyer flask.</li><br />
<li>Add 3g of agar in a flask wich contains BG-11.</li><br />
<li>Set pH at 7’4 for <i>Synechococcus elongatus</i> to come from Harvard. In the other hand, set the pH at 8’9 for <i>S. elongatus</i> to come from Golden.</li><br />
<li>Autoclave at 121ºC during 20 minutes while label and prepare the plates.</li><br />
<li>Add the antibiotics. Cool BG-11 with the agar.</li><br />
<li>Fill BG-11 in the plates and leave until solidify.</li><br />
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