Team:University College London/Week12YanikaExp6

From 2012.igem.org

Analytical were prepared as per the following recipes in order to confirm DNA identity. Ligations 1, 2, 4, 5 correspond to the purified DNA described above. In addition, four controls were used for each digest. A sample was cut with one enzyme in order to linearise the DNA.


LIGATIONS: 1 2 4 5 CONTROLS 1 2 4 5
dH2O 3.5 3.5 3.5 3.5 4.5 4.5 4.5 4.5
NEB 1 1 1 1 1 1 1 1
DNA 3 3 3 3 3 3 3 3
BSA 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5
XBal 1 1 1 1 1 1 1 1
SpeI 1 1 1 1 0 0 0 0
TOTAL 10 10 10 10 10 10 10 10