Team:University College London/Week12YanikaExp6

From 2012.igem.org

(Difference between revisions)
(Created page with "Analytical were prepared as per the following recipes in order to confirm DNA identity. Ligations 1, 2, 4, 5 correspond to the purified DNA described above. In addition, four con...")
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{| class ="bigtable"
{| class ="bigtable"
|-
|-
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! !! !! LIGATIONS !! !! !! !! CONTROLS !! !!  
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! !! LIGATIONS !! !! !! !! !! CONTROLS !!  !! !! !!
|-
|-
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| - || 1 || 2 || 4 || 5 || 1 || 2 || 4 || 5
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| - || || 1 || 2 || 4 || 5 || || 1 || 2 || 4 || 5
|-
|-
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|dH2O || 3.5 || 3.5 || 3.5 || 3.5 || 4.5 || 4.5 || 4.5 || 4.5
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|dH2O || || 3.5 || 3.5 || 3.5 || 3.5 || || 4.5 || 4.5 || 4.5 || 4.5
|-
|-
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|NEB || 1 || 1 || 1 || 1 || 1 || 1 || 1 || 1
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|NEB || || 1 || 1 || 1 || 1 || || 1 || 1 || 1 || 1
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|-
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|DNA || 3 || 3 || 3 || 3 || 3 || 3 || 3 || 3
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|DNA || || 3 || 3 || 3 || 3 || || 3 || 3 || 3 || 3
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|-
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|BSA || 0.5 || 0.5 || 0.5 || 0.5 || 0.5 || 0.5 || 0.5 || 0.5
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|BSA || || 0.5 || 0.5 || 0.5 || 0.5 || || 0.5 || 0.5 || 0.5 || 0.5
|-
|-
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|XBal || 1 || 1 || 1 || 1 || 1 || 1 || 1 || 1
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|XBal || || 1 || 1 || 1 || 1 || || 1 || 1 || 1 || 1
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|-
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|SpeI || 1 || 1 || 1 || 1 || 0 || 0 || 0 || 0
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|SpeI || || 1 || 1 || 1 || 1 || || 0 || 0 || 0 || 0
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|-
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|TOTAL || 10 || 10 || 10 || 10 || 10 || 10 || 10 || 10
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|TOTAL || || 10 || 10 || 10 || 10 || || 10 || 10 || 10 || 10
|}
|}

Revision as of 22:06, 26 September 2012

Analytical were prepared as per the following recipes in order to confirm DNA identity. Ligations 1, 2, 4, 5 correspond to the purified DNA described above. In addition, four controls were used for each digest. A sample was cut with one enzyme in order to linearise the DNA.


LIGATIONS CONTROLS
- 1 2 4 5 1 2 4 5
dH2O 3.5 3.5 3.5 3.5 4.5 4.5 4.5 4.5
NEB 1 1 1 1 1 1 1 1
DNA 3 3 3 3 3 3 3 3
BSA 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5
XBal 1 1 1 1 1 1 1 1
SpeI 1 1 1 1 0 0 0 0
TOTAL 10 10 10 10 10 10 10 10
Retrieved from "http://2012.igem.org/Team:University_College_London/Week12YanikaExp6"