Team:University College London/Week12YanikaExp6
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YanikaBorg (Talk | contribs) (Created page with "Analytical were prepared as per the following recipes in order to confirm DNA identity. Ligations 1, 2, 4, 5 correspond to the purified DNA described above. In addition, four con...") |
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{| class ="bigtable" | {| class ="bigtable" | ||
|- | |- | ||
- | ! !! !! | + | ! !! LIGATIONS !! !! !! !! !! CONTROLS !! !! !! !! |
|- | |- | ||
- | | - || 1 || 2 || 4 || 5 || 1 || 2 || 4 || 5 | + | | - || || 1 || 2 || 4 || 5 || || 1 || 2 || 4 || 5 |
|- | |- | ||
- | |dH2O || 3.5 || 3.5 || 3.5 || 3.5 || 4.5 || 4.5 || 4.5 || 4.5 | + | |dH2O || || 3.5 || 3.5 || 3.5 || 3.5 || || 4.5 || 4.5 || 4.5 || 4.5 |
|- | |- | ||
- | |NEB || 1 || 1 || 1 || 1 || 1 || 1 || 1 || 1 | + | |NEB || || 1 || 1 || 1 || 1 || || 1 || 1 || 1 || 1 |
|- | |- | ||
- | |DNA || 3 || 3 || 3 || 3 || 3 || 3 || 3 || 3 | + | |DNA || || 3 || 3 || 3 || 3 || || 3 || 3 || 3 || 3 |
|- | |- | ||
- | |BSA || 0.5 || 0.5 || 0.5 || 0.5 || 0.5 || 0.5 || 0.5 || 0.5 | + | |BSA || || 0.5 || 0.5 || 0.5 || 0.5 || || 0.5 || 0.5 || 0.5 || 0.5 |
|- | |- | ||
- | |XBal || 1 || 1 || 1 || 1 || 1 || 1 || 1 || 1 | + | |XBal || || 1 || 1 || 1 || 1 || || 1 || 1 || 1 || 1 |
|- | |- | ||
- | |SpeI || 1 || 1 || 1 || 1 || 0 || 0 || 0 || 0 | + | |SpeI || || 1 || 1 || 1 || 1 || || 0 || 0 || 0 || 0 |
|- | |- | ||
- | |TOTAL || 10 || 10 || 10 || 10 || 10 || 10 || 10 || 10 | + | |TOTAL || || 10 || 10 || 10 || 10 || || 10 || 10 || 10 || 10 |
|} | |} |
Revision as of 22:06, 26 September 2012
Analytical were prepared as per the following recipes in order to confirm DNA identity. Ligations 1, 2, 4, 5 correspond to the purified DNA described above. In addition, four controls were used for each digest. A sample was cut with one enzyme in order to linearise the DNA.
LIGATIONS | CONTROLS | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
- | 1 | 2 | 4 | 5 | 1 | 2 | 4 | 5 | ||
dH2O | 3.5 | 3.5 | 3.5 | 3.5 | 4.5 | 4.5 | 4.5 | 4.5 | ||
NEB | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
DNA | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | ||
BSA | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | ||
XBal | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
SpeI | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | ||
TOTAL | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |