Team:University College London/Week12YanikaExp5


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The first step was to design and construct six overlapping oligos. These were added in a 1:1:1:1:1:1 ratio with each primer having a working concentration of 100pmol/uL. The following recipe was prepared:

Ingredient Amount
20uL of each of the 6 primers 120ul
10X annealling buffer 15uL
dH20 15uL
TOTAL 150µl
100X BSA 0.5 µl
H2O to 50 µl

10X annealing buffer can be prepared using the following:

- 10mM EDTA

- 0.5M Tris HCl pH 8.0

- 0.1M MgCl2

- 0.5M NaCl