Team:University College London/Week12YanikaExp5

From 2012.igem.org

(Difference between revisions)
YanikaBorg (Talk | contribs)
(Created page with "The first step was to design and construct six overlapping oligos. These were added in a 1:1:1:1:1:1 ratio with each primer having a working concentration of 100pmol/uL. The foll...")
Newer edit →

Revision as of 21:26, 26 September 2012

The first step was to design and construct six overlapping oligos. These were added in a 1:1:1:1:1:1 ratio with each primer having a working concentration of 100pmol/uL. The following recipe was prepared:


Ingredient Amount
20uL of each of the 6 primers 120ul
10X annealling buffer 15uL
dH20 15uL
TOTAL 150µl
100X BSA 0.5 µl
H2O to 50 µl


10X annealing buffer can be prepared using the following:


- 10mM EDTA - 0.5M Tris HCl pH 8.0 - 0.1M MgCl2 - 0.5M NaCl