Team:University College London/Week12Yanika

From 2012.igem.org

1. In preparation, the night before, a colony was picked into 10ml of LB plus 10ul CMP

2. After, five dilutions were made from the sample, as follows:

Solution Ingredients
1 1ml of the O/N culture & 4ml of selective LB
2 1ml of the solution 1 & 4ml of the LB
3 1ml of the solution 2 & 4ml of the LB
4 1ml of the solution 3 & 4ml of the LB
5 1ml of the solution 4 & 4ml of the LB