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Team:University College London/Protocols/RestrictionEnzymeDigest1
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'''Step 4 - Centrifuge:''' To centrifuge, add sample eppendorf + balancer (fill another tube with equal volume of water if only have one sample) and screw lid on tight | '''Step 4 - Centrifuge:''' To centrifuge, add sample eppendorf + balancer (fill another tube with equal volume of water if only have one sample) and screw lid on tight | ||
| - | '''Step 5 - Digest Program:''' Then run on the following conditions | + | '''Step 5 - Digest Program:''' Then run on the following conditions |
RPM: 14000 | RPM: 14000 | ||
Revision as of 08:50, 1 August 2012
Enzyme Digest
Step 1 - Thawing cells: Thaw all materials on ice
Step 2 - Adding Ingredient: Add the following ingredients to autoclaved/sterile eppendorf tubes
Insert Table
Step 3 - Addition of BioBrick: Flick contents gently and centrifuge.
Step 4 - Centrifuge: To centrifuge, add sample eppendorf + balancer (fill another tube with equal volume of water if only have one sample) and screw lid on tight
Step 5 - Digest Program: Then run on the following conditions
RPM: 14000
Time: 1 minute
Temperature: 18oC
