http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Protocols/PrimerDesign&feed=atom&action=historyTeam:University College London/Protocols/PrimerDesign - Revision history2024-03-28T08:29:20ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Protocols/PrimerDesign&diff=78278&oldid=prevRwilkinson: /* Primer Design */2012-08-22T10:13:27Z<p><span class="autocomment">Primer Design</span></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 10:13, 22 August 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Primers should be designed to: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Primers should be designed to: </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1) Be 18-25 nucleotides in length</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1) Be 18-25 nucleotides in length</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2) Have a melting temperature that does not differ by more than 5oC between the two primers of a pair</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2) Have a melting temperature that does not differ by more than 5oC between the two primers of a pair</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3) GC nucleotides should constitute 40-60%</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3) GC nucleotides should constitute 40-60%</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4) Have a 3’primer end that is not complimentary to any region of the primer pair</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4) Have a 3’primer end that is not complimentary to any region of the primer pair</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>5) Contain an equal distribution of the nucleotides</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>5) Contain an equal distribution of the nucleotides</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>6) Closely match the target sequence (and so known polymorphic sites are avoided)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>6) Closely match the target sequence (and so known polymorphic sites are avoided)</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>7) Have a GC clamp on either end</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>7) Have a GC clamp on either end</div></td></tr>
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</table>Rwilkinsonhttp://2012.igem.org/wiki/index.php?title=Team:University_College_London/Protocols/PrimerDesign&diff=78277&oldid=prevRwilkinson: Created page with "<noinclude>{{:Team:University_College_London/templates/head|coverpicture=week4}} == Primer Design == </noinclude> Primers should be designed to: 1) Be 18-25 nucleotides in len..."2012-08-22T10:13:11Z<p>Created page with "<noinclude>{{:Team:University_College_London/templates/head|coverpicture=week4}} == Primer Design == </noinclude> Primers should be designed to: 1) Be 18-25 nucleotides in len..."</p>
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== Primer Design ==<br />
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Primers should be designed to: <br />
1) Be 18-25 nucleotides in length<br />
2) Have a melting temperature that does not differ by more than 5oC between the two primers of a pair<br />
3) GC nucleotides should constitute 40-60%<br />
4) Have a 3’primer end that is not complimentary to any region of the primer pair<br />
5) Contain an equal distribution of the nucleotides<br />
6) Closely match the target sequence (and so known polymorphic sites are avoided)<br />
7) Have a GC clamp on either end<br />
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