Team:University College London/Protocols/CurliCharacterisation


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Curli Characterisation

Step 1 - Prepare: Set out the Congo Red plates already prepared.

Step 2 - Streak Cells: Streak a sample of control cells (cells not expressing Curli) onto one antiobiotic-free congo red plate, and onto one antibiotic-inoculated congo red plate. Do the same for the curli-expressing cell line.

Step 3 - Incubate: Incubate cells overnight at 37oC or over the weekend at 30oC.