Team:University College London/Protocols/ColonyDNAExtraction

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(Difference between revisions)
(DNA Extraction from Colonies)
 
Line 13: Line 13:
Time: 1 min
Time: 1 min
 +
RPM: 8000
RPM: 8000
 +
Temp: 18oC
Temp: 18oC

Latest revision as of 09:42, 22 August 2012

DNA Extraction from Colonies

Step 1 - Picking Colony: Using a pipette select an isolated colony

Step 2 - Deposit: Dip the colony in 10ul of dH20, preferably in screw top container

Step 3 - Boiling Stage:Boil the dH20 100 for 5-10mins

Step 4 - Centrifuge:

Time: 1 min

RPM: 8000

Temp: 18oC

Step 5 - Remove Supernatant: The supernatant contains the DNA template.