Team:University College London/Protocols/ColonyDNAExtraction

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(DNA Extraction from Colonies)
 
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<noinclude>{{:Team:University_College_London/templates/head|coverpicture=week4}}
<noinclude>{{:Team:University_College_London/templates/head|coverpicture=week4}}
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== PCR ==
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== DNA Extraction from Colonies ==
</noinclude>
</noinclude>
'''Step 1 - Picking Colony:''' Using a pipette select an isolated colony
'''Step 1 - Picking Colony:''' Using a pipette select an isolated colony
 +
'''Step 2 - Deposit:''' Dip the colony in 10ul of dH20, preferably in screw top container
'''Step 2 - Deposit:''' Dip the colony in 10ul of dH20, preferably in screw top container
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'''Step 3 - Boiling Stage:'''Boil the dH20 100 for 5-10mins
'''Step 3 - Boiling Stage:'''Boil the dH20 100 for 5-10mins
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'''Step 4 - Centrifuge:'''
'''Step 4 - Centrifuge:'''
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Time: 1 min
Time: 1 min
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RPM: 8000
RPM: 8000
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Temp: 18oC
Temp: 18oC
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'''Step 5 - Remove Supernatant:''' The supernatant contains the DNA template.
'''Step 5 - Remove Supernatant:''' The supernatant contains the DNA template.
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Latest revision as of 09:42, 22 August 2012

DNA Extraction from Colonies

Step 1 - Picking Colony: Using a pipette select an isolated colony

Step 2 - Deposit: Dip the colony in 10ul of dH20, preferably in screw top container

Step 3 - Boiling Stage:Boil the dH20 100 for 5-10mins

Step 4 - Centrifuge:

Time: 1 min

RPM: 8000

Temp: 18oC

Step 5 - Remove Supernatant: The supernatant contains the DNA template.