Team:University College London/Protocols/ColPic

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(Picking Colonies)
 
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== Picking Colonies ==
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== Picking Colonies ==</noinclude>
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'''Step 1 – Creating culture media:''' In a sterile environment, set up X numbers of falcons, each with 5mls of media.  
'''Step 1 – Creating culture media:''' In a sterile environment, set up X numbers of falcons, each with 5mls of media.  
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'''Step 2 – Adding Antibiotic:''' Add Yul of antibiotic to reach desired antibiotic concentration.  
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'''Step 2 - Inoculating Colonies into a Selective Broth::''' Add Yul of antibiotic to reach desired antibiotic concentration.  
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'''Step 5 -'''  Culture your falcon tubes overnight at a temperature of 37 oC. Leave for no longer than 16 hours.
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'''Step 5 - Culture:'''  Culture your falcon tubes overnight at a temperature of 37 oC. Leave for no longer than 16 hours.
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Latest revision as of 15:21, 30 August 2012

Picking Colonies

Step 1 – Creating culture media: In a sterile environment, set up X numbers of falcons, each with 5mls of media.


Step 2 - Inoculating Colonies into a Selective Broth:: Add Yul of antibiotic to reach desired antibiotic concentration.


(For Ampicillin this is 50ug/ml, For Kanamycin it is 25ug/ml, for Tetracycline it is 15ug/ml, and for Chloramphenicol it is 25ug/ml)


Step 4 – Selecting a Colony: Select a clear, isolated colony and using an inoculation hoop scoop up a colony onto the tip. Deposit in the falcon tube


Step 5 - Culture: Culture your falcon tubes overnight at a temperature of 37 oC. Leave for no longer than 16 hours.