Team:University College London/Notebook/Week4

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Monday

Meeting – Construction/Characterisation We had meeting with our main supervisor Darren Nesbeth to discuss our progress on construction/characterisation and to set realistic aims for the week. Several aspects of construction were discussed. Main focus was on the detection module which was based on the human oestrogen receptor (ER). From the data collected on ER folding and activity in prokaryotic cells it became clear that it is extremely difficult to make ER function in prokaryotic cells. Consequently, we decided to look for alternatives for our detection system. However, as we already have ER on our kit we decided to evaluate its functionality at the end of the week to see if our assumptions about it were correct.