Notebook: Week 13
Preparations | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16
Aims of the Week
Commence characterisation for the laccase BioBrick. Prelim curli characterisation (II). Improve presentation and present to DN&EKM Friday. Predict required density/mass of bacteria in terms of containment and degradation modules - requires further experimental data (containment) and elaboration of Simbiology degradation model. Talk to UNCLOS guy and become a legal expert. skype LMU Munich (metabolic flux modelling) and Bielefeld (laccase modelling). Draft of metabolic flux modelling for irrE. Characterisation of our nuclease biobrick (compare with the results from Nuw cells nuclease). Prepare more competent cells, as we are doing tons of transformations, and the ligations are on fire!
Monday 3rd September
Bielefeld Collaboration Aurelija corresponded with Sebastien from iGEM Bielefeld to discuss how we are both modelling our use of laccase.
Hackspace Yeping finished planning the workshops for the collaboration with the London Hackspace. We're really looking forward to the workshops starting on Wednesday!
Tuesday 4th September
Darmstadt Collaboration Bouran swapped address with Henrik from Darmstadt today, we'll be sending them Laccase for characetrisation, while they are sending us a cutinase enzyme for expression in our Roseobacter.
Purdue Collaboration James was in touch with Chris from Purdue again to discuss swapping protocols for curli characteristion.
Lab clean-up Safety always first. We had a huge clean-up of the lab, including scrubbing the microwave spotless! We're now ready for our last month in the lab.
Wednesday 5th September
British Science Association Philipp and Bethan met with Roland Jackson to discuss our DIYbio collaboration.
Hackspace Day 1 We went en masse to the London Hackspace this evening for the first workshop. Carina, Yeping, Philipp, Martina and Bethan were all present. Howard met us there.
iGEM connection' Bethan is organising with iGEM Paris-Bettencourt a trip to Paris to discuss how our two project approach biosafety, and also discuss aspects of our DIYbio collaboration with members of the CRI's Education X.0 project. Paris iGEM also invited us to participate in their debate on the 16th!
Thursday 6th September
Hackspace Day 2 Together with the hackers, Bethan helped build a shaker to agitate our cells overnight.
Friday 7th September
Hackspace Day 3 Our shaker from yesterday was still alive today!
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