Team:University College London/Notebook/Week13

From 2012.igem.org

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== Monday 3rd September ==
== Monday 3rd September ==
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<div class="notebook-meeting"> '''Bielefeld Collaboration''' Aurelija corresponded with Sebastien from iGEM Bielefeld to discuss how we are both modelling our use of laccase.</div>
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<div class="notebook-meeting"> '''Hackspace Day 1''' - Martina, Leonard and Yeping worked with William, Tonderai and Simon. Philipp took lots of pictures! </div>
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<div class="notebook-meeting"> '''Hackspace''' Yeping finished planning the workshops for the collaboration with the London Hackspace. We're really looking forward to the workshops starting on Wednesday! </div>
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<div class="notebook-meeting"> '''LMU Munich Collaboration''' - Aurelija skyped with Simon and Julia from iGEM Munich (LMU).</div>
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<div class="notebook-meeting"> '''Hackspace Exhibition''' - Philipp and Howard met today to find a location for our exhibit. </div>
== Tuesday 4th September ==
== Tuesday 4th September ==
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<div class="notebook-meeting"> '''Darmstadt Collaboration''' Bouran swapped address with Henrik from Darmstadt today, we'll be sending them Laccase for characetrisation, while they are sending us a cutinase enzyme for expression in our Roseobacter.</div>
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<div class="notebook-meeting"> '''Hackspace Day 2''' - The hackers, Yeping and Martina ran a second gel today as well as doing PCR. </div>
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<div class="notebook-meeting"> '''Purdue Collaboration''' James was in touch with Chris from Purdue again to discuss swapping protocols for curli characteristion. </div>
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<div class="notebook-meeting"> '''Lab clean-up''' Safety always first. We had a huge clean-up of the lab, including scrubbing the microwave spotless! We're now ready for our last month in the lab. </div>
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== Wednesday 5th September ==
== Wednesday 5th September ==
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<div class="notebook-meeting"> '''British Science Association''' Philipp and Bethan met with Roland Jackson to discuss our DIYbio collaboration. </div>
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<div class="notebook-meeting"> '''Hackspace Day 1''' We went en masse to the London Hackspace this evening for the first workshop. Carina, Yeping, Philipp, Martina and Bethan were all present. Howard met us there.  </div>
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<div class="notebook-meeting"> '''Hackspace Day 3''' </div>
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<div class="notebook-meeting"> ''iGEM connection''' Bethan is organising with iGEM Paris-Bettencourt a trip to Paris to discuss how our two project approach biosafety, and also discuss aspects of our DIYbio collaboration with members of the CRI's Education X.0 project. Paris iGEM also invited us to participate in their debate on the 16th! </div>
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== Thursday 6th September ==
== Thursday 6th September ==
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<div class="notebook-meeting"> '''Hackspace Day 2''' Together with the hackers, Bethan helped build a shaker to agitate our cells overnight. </div>
 
== Friday 7th September ==
== Friday 7th September ==
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<div class="notebook-meeting"> '''Hackspace Day 3''' Our shaker from yesterday was still alive today! </div>
 
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<div class="notebook-meeting"> '''Press''' C-lab published an exciting write-up of our 1st day at the Hackspace http://c-lab.co.uk/blogs/visiting-london-hackspace-with-ucl-igem.html</div>
 
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Revision as of 15:09, 4 September 2012

Contents

Notebook: Week 13

Preparations | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16


Aims of the Week

Commence characterisation for the laccase BioBrick. Prelim curli characterisation (II). Improve presentation and present to DN&EKM Friday. Predict required density/mass of bacteria in terms of containment and degradation modules - requires further experimental data (containment) and elaboration of Simbiology degradation model. Talk to UNCLOS guy and become a legal expert. skype LMU Munich (metabolic flux modelling) and Bielefeld (laccase modelling). Draft of metabolic flux modelling for irrE. Characterisation of our nuclease biobrick (compare with the results from Nuw cells nuclease). Prepare more competent cells, as we are doing tons of transformations, and the ligations are on fire!

Monday 3rd September

Hackspace Day 1 - Martina, Leonard and Yeping worked with William, Tonderai and Simon. Philipp took lots of pictures!
LMU Munich Collaboration - Aurelija skyped with Simon and Julia from iGEM Munich (LMU).
Hackspace Exhibition - Philipp and Howard met today to find a location for our exhibit.

Tuesday 4th September

Hackspace Day 2 - The hackers, Yeping and Martina ran a second gel today as well as doing PCR.

Wednesday 5th September

Hackspace Day 3

Thursday 6th September

Friday 7th September