Team:University College London/Notebook/Week12

From 2012.igem.org

Revision as of 23:30, 9 September 2012 by Yeping (Talk | contribs)

Contents

Notebook: Week 12

Preparations | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16


Aims of the Week

This week we're starting the workshops in collaboration with the DIYbio group, we'll be running a series of workshops in the London Hackspace at the end of the week.

Monday 27th August

Bielefeld Collaboration Aurelija corresponded with Sebastien from iGEM Bielefeld to discuss how we are both modelling our use of laccase.
Hackspace Yeping finished planning the workshops for the collaboration with the London Hackspace. We're really looking forward to the workshops starting on Wednesday!

Tuesday 28th August

Darmstadt Collaboration Bouran swapped address with Henrik from Darmstadt today, we'll be sending them Laccase for characetrisation, while they are sending us a cutinase enzyme for expression in our Roseobacter.
Purdue Collaboration James was in touch with Chris from Purdue again to discuss swapping protocols for curli characteristion.
Lab clean-up Safety always first. We had a huge clean-up of the lab, including scrubbing the microwave spotless! We're now ready for our last month in the lab.

Wednesday 29th August

British Science Association Philipp and Bethan met with Roland Jackson to discuss our DIYbio collaboration.
Hackspace Day 1 We went en masse to the London Hackspace this evening for the first workshop. Carina, Yeping, Philipp, Martina and Bethan were all present. Howard met us there.
iGEM connection' Bethan is organising with iGEM Paris-Bettencourt a trip to Paris to discuss how our two project approach biosafety, and also discuss aspects of our DIYbio collaboration with members of the CRI's Education X.0 project. Paris iGEM also invited us to participate in their debate on the 16th!

Thursday 30th August

Hackspace Day 2 Together with the hackers, Bethan helped build a shaker to agitate our cells overnight.

Friday 31st August

Hackspace Day 3 Our shaker from yesterday was still alive today! Together with the biohackers we ran a gel using their DIY gel tray of the PCR product from yesterday. Using a hand held UV filter and UV light, we visualized our gel. The PCR has worked! Gel shows the expected band at 2070bp.
Press C-lab published an exciting write-up of our 1st day at the Hackspace