Team:University College London/Module 6/Results


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Module 6: Containment

Description | Design | Construction | Characterisation | Modelling | Results | Conclusions

DNase Agar Assay

The following table shows the results we obtained from the DNase agar test. DNase agar contains DNA, which our extracellular nuclease digests. This digestion is observed by adding hydrochloric acid to the agar plates, which causes the remaining DNA to precipitate, causing 'cloudiness' to the DNase agar.

As seen in our DNase agar plate, there is a clear halo surrounding our nuclease transformed cells, indicating a DNA-free zone where the nuclease has digested the DNA in the agar. No such halo is present in our untransformed cells. This indicates that our transformation has been successful, and that BBa_K729004 is working as expected, producing extracellular nuclease that is capable of digesting extracellular genetic material.

DNase Agar Assay
Control BBa_K729019
UniversityCollegeLondon Nuclease Control.jpg UniversityCollegeLondon Nuclease K729004.jpg

Colony Halo size Assay

A separate DNase agar assay was also done, in order to determine if there was any correlation between colony size and nuclease production of our nuclease BioBrick (BBa_K729019)

UniversityCollegeLondon Nuclease Colony Halo Diameter correlation.png

Nuclease Toxicity Assay

UniversityCollegeLondon Nuclease Toxicity Growth Curve Assay.png