Team:University College London/Module 6/Characterisation


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Module 6: Containment

Description | Design | Construction | Characterisation | Modelling | Results | Conclusions


"The days of the head-shaking dismissal of the questions concerning risks, and the easy answers referring to the vast experience from the laboratory experiments are over; they have been replaced by scientific investigations of the questions and increased activity in the field of ecology. This is an interesting example of scientific choice being influenced by events in the rest of the society." (Molin et al. 1993)

In order to determine the activity of the exonuclease, we will be utilising DNAse agar. This agar contains DNA fragments that would be cleaved by the exonuclease, leaving the regions surrounding the colonies free of genetic material. The agar plates can then be stained with a DNA indicator such as Hydrochloric Acid or Toludine Blue, allowing the DNA voids in the agar to be visible.

For the toxin/anti-toxin pairs characterisation, we will be transforming our toxic plasmids into cell both with and without the genomic anti-toxin genes. By comparing the ratio of cell viability in each case, we will be able to identify how effective our containment system is.