http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Module_4/Results&feed=atom&action=historyTeam:University College London/Module 4/Results - Revision history2024-03-28T23:27:35ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Module_4/Results&diff=229976&oldid=prevBayke5: /* Expression Essays */2012-09-27T01:25:59Z<p><span class="autocomment">Expression Essays</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_K200018.jpg]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_K200018.jpg<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_K729008.jpg]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_K729008.jpg<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This chart shows the fluorescence of GFP from module (BBa_K729008) that integrates the T7 RNA polymerase and T7 promoter in order to increase the expression of the reporter gene. Once the bacteria is exposed to carbon starvation stress, cstA promoter is activated, as a results it can be seen from the chart that GFP was expressed reaching higher accumulation in cultures with M9 media supplemented with less than 0.5% of glucose. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This chart shows the fluorescence of GFP from module (BBa_K729008) that integrates the T7 RNA polymerase and T7 promoter in order to increase the expression of the reporter gene. Once the bacteria is exposed to carbon starvation stress, cstA promoter is activated, as a results it can be seen from the chart that GFP was expressed reaching higher accumulation in cultures with M9 media supplemented with less than 0.5% of glucose. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_Comparison.jpg]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_Comparison.jpg<ins class="diffchange diffchange-inline">|center</ins>]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The chart shows GFP fluorescence profiles for different constructions. In order to demonstrate that our new biobrick increases the output production when using the cstA promoter and T7 RNA polymerase, we compared our results with other modules using minimal M9 media supplemented with 0.2% glucose: pcstA-GFP (BBa_K200018), pT7-GFP (BBa_I746909) and a constitutive promoter fused with GFP (BBa_I20260). From our results ''E. coli'' transformed with our module (BBa_K729008) demonstrated a 3 fold increase in GFP expression over the BBa_K200018 (pcstA-GFP) developed by Imperial College’s 2009 iGem team.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The chart shows GFP fluorescence profiles for different constructions. In order to demonstrate that our new biobrick increases the output production when using the cstA promoter and T7 RNA polymerase, we compared our results with other modules using minimal M9 media supplemented with 0.2% glucose: pcstA-GFP (BBa_K200018), pT7-GFP (BBa_I746909) and a constitutive promoter fused with GFP (BBa_I20260). From our results ''E. coli'' transformed with our module (BBa_K729008) demonstrated a 3 fold increase in GFP expression over the BBa_K200018 (pcstA-GFP) developed by Imperial College’s 2009 iGem team.</div></td></tr>
</table>Bayke5http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Module_4/Results&diff=201600&oldid=prevBayke5: /* Expression Essays */2012-09-26T15:24:07Z<p><span class="autocomment">Expression Essays</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We carried out </del>further characterization of the pcstA fused with GFP (BBa_K200018) built by Imperial College's 2009 iGem team. Fluorescence was measured from overnight cultures and data collected shows significant higher expression for cultures with 0%, 0.2%, 0.5% glucose concentration, this essay also shows how glucose represses cstA promoter.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The graph above show the </ins>further characterization of the pcstA fused with GFP (BBa_K200018) built by Imperial College's 2009 iGem team. Fluorescence was measured from overnight cultures and data collected shows significant higher expression for cultures with 0%, 0.2%, 0.5% glucose concentration, this essay also shows how glucose represses cstA promoter.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Our </del>module (BBa_K729008) integrates the T7 RNA polymerase and T7 promoter in order to increase the expression of the reporter gene. Once the bacteria is exposed to carbon starvation stress, cstA promoter is activated, as a results it can be seen from the chart that GFP was expressed reaching higher accumulation in cultures with M9 media supplemented with less than 0.5% of glucose. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">This chart shows the fluorescence of GFP from </ins>module (BBa_K729008) <ins class="diffchange diffchange-inline">that </ins>integrates the T7 RNA polymerase and T7 promoter in order to increase the expression of the reporter gene. Once the bacteria is exposed to carbon starvation stress, cstA promoter is activated, as a results it can be seen from the chart that GFP was expressed reaching higher accumulation in cultures with M9 media supplemented with less than 0.5% of glucose. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_Comparison.jpg]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_Comparison.jpg]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In order to demonstrate that our new biobrick increases the output production when using the cstA promoter and T7 RNA polymerase, we compared our results with other modules using minimal M9 media supplemented with 0.2% glucose: pcstA-GFP (BBa_K200018), pT7-GFP (BBa_I746909) and a constitutive promoter fused with GFP (BBa_I20260). ''E. coli'' transformed with our module (BBa_K729008) demonstrated a 3 fold increase in GFP expression over the BBa_K200018 (pcstA-GFP) developed by Imperial College’s 2009 iGem team.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The chart shows GFP fluorescence profiles for different constructions. </ins>In order to demonstrate that our new biobrick increases the output production when using the cstA promoter and T7 RNA polymerase, we compared our results with other modules using minimal M9 media supplemented with 0.2% glucose: pcstA-GFP (BBa_K200018), pT7-GFP (BBa_I746909) and a constitutive promoter fused with GFP (BBa_I20260). <ins class="diffchange diffchange-inline">From our results </ins>''E. coli'' transformed with our module (BBa_K729008) demonstrated a 3 fold increase in GFP expression over the BBa_K200018 (pcstA-GFP) developed by Imperial College’s 2009 iGem team.</div></td></tr>
</table>Bayke5http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Module_4/Results&diff=197511&oldid=prevBayke5: /* Expression Essays */2012-09-26T13:35:06Z<p><span class="autocomment">Expression Essays</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Expression Essays==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Expression Essays==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli <del class="diffchange diffchange-inline">carrying BBa_K200018 </del>was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%). </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. <ins class="diffchange diffchange-inline">Transformed ''</ins>E. coli<ins class="diffchange diffchange-inline">'' </ins>was grown <ins class="diffchange diffchange-inline">overnight </ins>in <ins class="diffchange diffchange-inline">LB media and then transferred into </ins>minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%) <ins class="diffchange diffchange-inline">to induce the activation of the cstA promoter</ins>. </div></td></tr>
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</table>Bayke5http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Module_4/Results&diff=197378&oldid=prevBayke5: /* Characterization */2012-09-26T13:31:24Z<p><span class="autocomment">Characterization</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==<del class="diffchange diffchange-inline">Characterization</del>==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==<ins class="diffchange diffchange-inline">Expression Essays</ins>==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%). </div></td></tr>
</table>Bayke5http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Module_4/Results&diff=197327&oldid=prevBayke5 at 13:30, 26 September 20122012-09-26T13:30:18Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Characterization==</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%). </div></td></tr>
</table>Bayke5http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Module_4/Results&diff=197278&oldid=prevBayke5: /* Characterisation of Starvation Promoter BBa_K118011 */2012-09-26T13:28:50Z<p><span class="autocomment">Characterisation of Starvation Promoter BBa_K118011</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">== Characterisation of Starvation Promoter BBa_K118011 ==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%). </div></td></tr>
</table>Bayke5http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Module_4/Results&diff=197225&oldid=prevBayke5: /* Characterisation of Starvation Promoter BBa_K118011 */2012-09-26T13:27:23Z<p><span class="autocomment">Characterisation of Starvation Promoter BBa_K118011</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We carried out further characterization of the pcstA fused with GFP (BBa_K200018) built by Imperial College's 2009 iGem team. Fluorescence was measured from overnight cultures and data collected shows significant higher expression for cultures with 0%, 0.2%, 0.5% glucose concentration, this essay also shows how glucose represses cstA promoter.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We carried out further characterization of the pcstA fused with GFP (BBa_K200018) built by Imperial College's 2009 iGem team. Fluorescence was measured from overnight cultures and data collected shows significant higher expression for cultures with 0%, 0.2%, 0.5% glucose concentration, this essay also shows how glucose represses cstA promoter.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_K729008.jpg]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_K729008.jpg]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our module (BBa_K729008) integrates the T7 RNA polymerase and T7 promoter in order to increase the expression of the reporter gene. Once the bacteria is exposed to carbon starvation stress, cstA promoter is activated, as a results it can be seen from the chart that GFP was expressed reaching higher accumulation in cultures with M9 media supplemented with less than 0.5% of glucose. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our module (BBa_K729008) integrates the T7 RNA polymerase and T7 promoter in order to increase the expression of the reporter gene. Once the bacteria is exposed to carbon starvation stress, cstA promoter is activated, as a results it can be seen from the chart that GFP was expressed reaching higher accumulation in cultures with M9 media supplemented with less than 0.5% of glucose. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_Comparison.jpg]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_Comparison.jpg]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to demonstrate that our new biobrick increases the output production when using the cstA promoter and T7 RNA polymerase, we compared our results with other modules using minimal M9 media supplemented with 0.2% glucose: pcstA-GFP (BBa_K200018), pT7-GFP (BBa_I746909) and a constitutive promoter fused with GFP (BBa_I20260). ''E. coli'' transformed with our module (BBa_K729008) demonstrated a 3 fold increase in GFP expression over the BBa_K200018 (pcstA-GFP) developed by Imperial College’s 2009 iGem team.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to demonstrate that our new biobrick increases the output production when using the cstA promoter and T7 RNA polymerase, we compared our results with other modules using minimal M9 media supplemented with 0.2% glucose: pcstA-GFP (BBa_K200018), pT7-GFP (BBa_I746909) and a constitutive promoter fused with GFP (BBa_I20260). ''E. coli'' transformed with our module (BBa_K729008) demonstrated a 3 fold increase in GFP expression over the BBa_K200018 (pcstA-GFP) developed by Imperial College’s 2009 iGem team.</div></td></tr>
</table>Bayke5http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Module_4/Results&diff=197206&oldid=prevBayke5: /* Characterisation of Starvation Promoter BBa_K118011 */2012-09-26T13:26:48Z<p><span class="autocomment">Characterisation of Starvation Promoter BBa_K118011</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_Comparison.jpg]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_Comparison.jpg]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In order to demonstrate that our <del class="diffchange diffchange-inline">construction </del>increases output production when using the cstA promoter, we compared our results with other modules using minimal M9 media supplemented with 0.2% glucose: pcstA-GFP (BBa_K200018), pT7-GFP (BBa_I746909) and a constitutive promoter fused with GFP (BBa_I20260). E. coli transformed with our module (BBa_K729008) demonstrated a 3 fold increase in GFP expression over the BBa_K200018 (pcstA-GFP) developed by Imperial College’s 2009 iGem team.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In order to demonstrate that our <ins class="diffchange diffchange-inline">new biobrick </ins>increases <ins class="diffchange diffchange-inline">the </ins>output production when using the cstA promoter <ins class="diffchange diffchange-inline">and T7 RNA polymerase</ins>, we compared our results with other modules using minimal M9 media supplemented with 0.2% glucose: pcstA-GFP (BBa_K200018), pT7-GFP (BBa_I746909) and a constitutive promoter fused with GFP (BBa_I20260). <ins class="diffchange diffchange-inline">''</ins>E. coli<ins class="diffchange diffchange-inline">'' </ins>transformed with our module (BBa_K729008) demonstrated a 3 fold increase in GFP expression over the BBa_K200018 (pcstA-GFP) developed by Imperial College’s 2009 iGem team.</div></td></tr>
</table>Bayke5http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Module_4/Results&diff=197131&oldid=prevBayke5: /* Characterisation of Starvation Promoter BBa_K118011 */2012-09-26T13:24:48Z<p><span class="autocomment">Characterisation of Starvation Promoter BBa_K118011</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_Comparison.jpg]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_Comparison.jpg]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">In order to demonstrate that our construction increases output production when using the cstA promoter, we compared our results with other modules using minimal M9 media supplemented with 0.2% glucose: pcstA-GFP (BBa_K200018), pT7-GFP (BBa_I746909) and a constitutive promoter fused with GFP (BBa_I20260). E. coli transformed with our module (BBa_K729008) demonstrated a 3 fold increase in GFP expression over the BBa_K200018 (pcstA-GFP) developed by Imperial College’s 2009 iGem team.</ins></div></td></tr>
</table>Bayke5http://2012.igem.org/wiki/index.php?title=Team:University_College_London/Module_4/Results&diff=197092&oldid=prevBayke5: /* Characterisation of Starvation Promoter BBa_K118011 */2012-09-26T13:23:46Z<p><span class="autocomment">Characterisation of Starvation Promoter BBa_K118011</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_K729008.jpg]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_K729008.jpg]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Our module (BBa_K729008) integrates the T7 RNA polymerase and T7 promoter in order to increase the expression of the reporter gene. Once the bacteria is exposed to carbon starvation stress, cstA promoter is activated, as a results it can be seen from the chart that GFP was expressed reaching higher accumulation in cultures with M9 media supplemented with less than 0.5% of glucose. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_Comparison.jpg]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:UniversityCollegeLondon_Buoyancy_Comparison.jpg]]</div></td></tr>
</table>Bayke5