Team:University College London/Module 3/Design


Revision as of 18:35, 23 August 2012 by Rwilkinson (Talk | contribs)

Module 3: Design

Description | Design | Construction | Characterisation | Modelling | Results | Conclusions



We identified two requirements for this system, and have designed the module to meet each requirement.

Requirement 1: The bacteria should carry a system capable of degrading polythelene

Laccase, an oxidoreductive enzyme, is important in bioremediation. Laccase from Rhodococcus ruber c208 has been found to be involved in polyethylene biodegradation ( The Laccase mechanism involves the generation of free radicals which encourages degradation of polyethylene. As Rhodococcus strains are presently unavailable to us, our system includes cueO, a laccase/multi-copper oxidase from E. coli.

Requirement 2: The system must be compatible with the marine environment

Our chosen enzyme has been suggested to survive in harsh conditions; laccase is thermostable ( and can function within a broad pH range, ( therefore we expect our system will function in the marine environment.