Team:University College London/Module 3/Design

From 2012.igem.org

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(Description)
(Description)
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'''Requirement 1: The bacteria should carry a system capable of degrading polythelene'''
'''Requirement 1: The bacteria should carry a system capable of degrading polythelene'''
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Laccase, an oxidoreductive enzyme, is important in bioremediation. Laccase from Rhodococcus ruber c208 has been found to be involved in polyethylene biodegradation (http://www.sciencedirect.com/science/article/pii/S0964830512000637). The Laccase mechanism involves the generation of free radicals which encourages degradation of polyethylene. As Rhodococcus strains are presently unavailable to us, our system includes cueO, a laccase/multi-copper oxidase from E. coli.  
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Laccase, an oxidoreductive enzyme, is important in bioremediation. Laccase from ''Rhodococcus Ruber'' C208 has been found to be involved in polyethylene biodegradation (http://www.sciencedirect.com/science/article/pii/S0964830512000637). The Laccase mechanism involves the generation of free radicals which encourages degradation of polyethylene. As Rhodococcus strains are presently unavailable to us, our system includes cueO, a laccase/multi-copper oxidase from E. coli.  
'''Requirement 2: The system must be compatible with the marine environment'''
'''Requirement 2: The system must be compatible with the marine environment'''
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Our chosen enzyme has been suggested to survive in harsh conditions; laccase is thermostable (http://www.ncbi.nlm.nih.gov/pubmed/14586105) and can function within a broad pH range, (http://www.sciencedirect.com/science/article/pii/S0960852412002234) therefore we expect our system will function in the marine environment.
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Our chosen enzyme has been suggested to survive in harsh conditions; Laccase is thermostable (http://www.ncbi.nlm.nih.gov/pubmed/14586105) and can function within a broad pH range, (http://www.sciencedirect.com/science/article/pii/S0960852412002234) therefore we expect our system will function in the marine environment.
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{{:Team:University_College_London/templates/foot}}

Revision as of 09:56, 24 August 2012

Module 3: Design

Description | Design | Construction | Characterisation | Modelling | Results | Conclusions

Description

Degradation

We identified two requirements for this system, and have designed the module to meet each requirement.

Requirement 1: The bacteria should carry a system capable of degrading polythelene

Laccase, an oxidoreductive enzyme, is important in bioremediation. Laccase from Rhodococcus Ruber C208 has been found to be involved in polyethylene biodegradation (http://www.sciencedirect.com/science/article/pii/S0964830512000637). The Laccase mechanism involves the generation of free radicals which encourages degradation of polyethylene. As Rhodococcus strains are presently unavailable to us, our system includes cueO, a laccase/multi-copper oxidase from E. coli.

Requirement 2: The system must be compatible with the marine environment

Our chosen enzyme has been suggested to survive in harsh conditions; Laccase is thermostable (http://www.ncbi.nlm.nih.gov/pubmed/14586105) and can function within a broad pH range, (http://www.sciencedirect.com/science/article/pii/S0960852412002234) therefore we expect our system will function in the marine environment.